Studies on Classification and Antiserotypes of Monoclonal Antibodies Against Pseudomonas Solanacearum
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摘要: 用甘薯青枯病菌(Pseudomonas solanacearcum)免疫的BALB/C小鼠脾细胞与鼠骨髓瘤细胞杂交融合,建立了4株分泌抗甘薯青枯病菌的特异性、有鉴别力的杂交瘤细胞株:PSB24、PSB28、PSB46、PSB56.杂交瘤细胞株染色体数范围为98～104,ELISA试验培养液抗体滴度为102～103,腹水抗体滴度为105～106.这些McAb的免疫球蛋白的亚类分别属于IgG1和IgM.这4株单抗分别与来自不同区域的156株甘薯青枯病菌进行酶联免疫吸附试验,结果表明:可将这些菌株识别出4个抗原决定簇,区分出7个血清型(Ⅰ～Ⅶ).在7个血清型中Ⅰ型和Ⅲ型在我省数量大、分布面广,是我省主要优势流行型;而Ⅶ型数量少、分布面小,可视为我省的一个弱势型。Abstract: Mouse marrow cell was hy bridized by immunized BA LB/C mouse spleen cell of pseudo monas solanacearum.Four hybridoma cell lines PSB24,PSB28,PSB46 and P SB56 ag ainst pseudo monas so lanacearum were established.The chromosomce numbers of the hybrido macell lines ranged from 98 to 104.The ELISA titers of the culture supenatants and the ascitic fluid were 102-103 and 105-106 respect ively.These McAbsbelonged to Ig G1 and IgMindividually.Each of these four McAbs was reacted individually with 157 pseudo monas solanacearum from different areas by ELISA.The results showed that four antigen deter minants and seven mono antisero types (McAbⅠ-Ⅶ)were distinguished from these strains.A mong them,the first type(Ⅰ)and the thir done(Ⅲ)is do minant types and large amount,distributed widely,but the seventh type(Ⅶ) was an undo minant one and small amount,distrbuted narrowly in Fujian province.
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