Constructing SSR Molecular Database for Identity System of Hybrid Rice Parents
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摘要: 利用前期构建的基于明恢86的SSR电子遗传图谱,筛选得到分布于水稻12条染色体长短臂上的24对多态性好的SSR标记,利用这些标记对139份水稻品种材料进行多态性分析,从而得到142个等位位点,24个标记位点的分布范围在2～10之间,平均值为5.9。这些标记对杂交稻组合的鉴别能力高于对杂交稻亲本的鉴别能力,这在对目前生产中数目繁多的杂交组合的识别鉴定具备突出的优势。对18个杂交稻组合与其亲本材料进行了标记匹配性分析,在确定了母本高精确性遗传的同时,剔除了2个假杂交稻。此外,构建了137个水稻品种材料×24个标记的分子身份证数据库,本库可直接应用于生产中相关的亲本鉴定及种子验杂,从而为杂交水稻品种纯度和真伪鉴定提供科学数据。Abstract: Based on previous construction of electron genetic map for MingHui 86 DNA micro-satellite markers(SSR),24 high polymorphism markers were obtained in distribution on all 12 rice chromosomes.By the markers,polymorphism analysis for 139 rice germplasm was conducted and 142 alleles were obtained with the 24 loci distributed in range between 2 to 10 and average at 5.9.The identifying capacity of the markers is higher for hybrid combinations than for hybrid rice parents.Eighteen hybrid combinations were compared with their parents by marker-matching analysis.In accordance with the identification in high genetic reliability of maternal lines,two false hybrid were eliminated from the sample base.A molecular identity database was constructed based on the137 rice materials with?24 marker loci,which could be directly applied for parental identification and miscellaneous seeds.
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[1] CHEN X,TEMNYKH S,XU Y,et al.Development of a micro-satellite framework map providing genome-wide coverage in rice (Oryza sativa L.)[J].Theor Appl Genet,1997,95(4):553-567. [2] 黄燕娟,华亚鹏,刘华清,等.三个基于水稻明恢86的SSR电子遗传图谱的构建[J].分子植物育种,2008,6(4):655-663. -
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