摘要:
从四种田菁根瘤分离出49L、50M、51S和53号四株快生型根瘤菌。这些菌株能产酸,能利用的糖类较多,能在pH5.5～9.0和含盐(NaCl)0.5%的培养基生长,最适的生长温度是25～30℃;53号菌株还可以在pH10和含盐1.0%的培养基于温度35℃下生长。从毛萼田菁茎瘤分离出54号慢生型菌株。此菌株能产碱,能利用的糖类较少,对pH适应幅度较小。这些菌株对宿主回接和互接均可结瘤固氮。田间种植的田菁根瘤有较高的固氮酶活性和氢酶活性。毛萼田菁的根瘤和茎瘤这两种酶活性更高。田菁根瘤菌抗逆性强。其寄主田菁是改良盐碱地的优良植物,毛萼田菁更好。
Abstract:
The rhizobial strains 49L, 50M and 51S, as well as 53 from the root nodules of four sesbania specieshave been isolated.They are fast-growing, which produce acid on yeast-mannitol agar and utilize most of carbohydrates as carbon Source.The optimum growth temperature is 25～30℃, grow well at pH 5.5～9.0 andtolerate to 0.5% NaCl.Strain 53 can also grow vigorously at 35℃ and PH 10, tolerate to 1.0% NaCl.Aslow-growing strain 54 from stem nodule of Sesbania rostrata has been isolated.On yeast-mannitol agar, it produces alkali substance.The pH range for its growth is 6～8, optimum is 7.0.It grows well at 25～30℃, tolerates to 1.0% NaCl and utilizes less carbohydrates.When inoculating or cross-inoculating, the host plantsformed nodules with high nitrogenase and hydrogenase activities.
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