Abstract: High male sterility rates ranged in 97.7%-99.5% were obtained by spraying the indica rice Yu B with 200 ppm R1 or 200 ppm R1 and 400 ppm E, and by spraying japonica rice Guandong 127, Xihai 172 with 100 ppm R1 and 200 ppm E in the late stage of spikelet differentiation and meiosis Phase.In the malesterilantes treated rice, the anther deformity, thickened tapetum, thin-walled cell lysis and blocked nutrition tract were found under scanning electron microscope; the microdiodange failed to form pollen, or formed few pollen and half of which were morphologically abortive.