摘要:
利用ISSR分子标记技术对24份白肉枇杷种质资源进行分析。从89条ISSR引物中筛选出16条多态性好、扩增清晰的引物,共获得195个位点,有153个多态性位点,多态性百分率78.46%。采用NTSYS-PC2.0分析软件进行聚类分析,24份白肉枇杷种质资源的遗传相似系数为0.55~0.89,在遗传系数为 0.72处将24份白肉枇杷种质资源划分为 3类。结果表明,白肉枇杷种间在分子水平上具有丰富的遗传多样性,供试白肉枇杷的亲缘关系与地理来源相关性不甚明显, 多数来源相同的种质资源遗传关系近,但也存在明显的不同来源地的种质资源聚为一类;同期检测到的7条特异性条带,可作为供试白肉枇杷的6个品种鉴定的参考性标记;获得的部分特异标记可以区分贵妃、新白1号和新白8号。
关键词:
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枇杷 /
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白肉 /
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种质资源 /
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ISSR /
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亲缘关系
Abstract:
Genetic relationship of 24 white flesh loquat germplasms was studied by ISSR technique. 16 primers which could produce clear and polymorphic bands were screened out from 89 ISSR random primers. 195 locis were detected, of which 153 were polymorphic and the polymorphic percentage was 78.46%. As analyzed by NTSYS-PC2.0, the genetic similarity ranged from 0.55-0.89.24 white-flesh loquat germplasms were grouped into three sub-cluster when the similar coefficient was 0.72. Rich genetic diversity of white-flesh loquat germplasm resources was detected at DNA level. in which relationship were not so obvious correlation with geographic origin. Most germplasm resources which came from the same geographic origin had close relationship, But some germplasm resources with different source regions were clustered in a group obviously. In addition, during the reserch we also found that 7 special bands could be reference mark of 6 Loquat cultivars identity. Some specific ISSR markers could identify Guifei, Xinbai No.1 and Xinbai No.8.
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