根癌农杆菌介导籼稻高效遗传转化体系的构建

许惠滨; 魏毅东; 连玲; 何炜; 张建福; 谢华安

根癌农杆菌介导籼稻高效遗传转化体系的构建

许惠滨^1,2,3,4,5,
魏毅东^1,2,3,4,5,
连玲^1,2,3,4,5,
何炜^1,2,3,4,5,
张建福^{1,2,3,4,5, ,},
谢华安^{1,2,3,4,5, ,}

1.
福建省农业科学院水稻研究所, 福建福州 350019;
2.
农业部华南杂交水稻种质创新与分子育种重点实验室/福州国家水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室, 福建福州 350003;
3.
福建省作物种质创新与分子育种省部共建国家重点实验室培育基地, 福建福州 350003;
4.
水稻国家工程实验室, 福建福州 350003;
5.
杂交水稻国家重点实验室华南基地, 福建福州 350003

基金项目:

国家转基因农作物新品种培育重大专项(2008ZX08001-006、2011ZX08001-006、2011ZX08001-004);福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y04)

详细信息

通讯作者:
张建福(1971- ).博士.副研究员.主要从事水稻遗传学与分子育种研究(E-mail:jianfzhang@163.com);谢华安(1911- ).研究员.中国科学院院士.主要从事水稻遗传育种研究(E-mail:huaanxie@yahoo,com,cn)

张建福(1971- ).博士.副研究员.主要从事水稻遗传学与分子育种研究(E-mail:jianfzhang@163.com);谢华安(1911- ).研究员.中国科学院院士.主要从事水稻遗传育种研究(E-mail:huaanxie@yahoo,com,cn)

中图分类号: S511
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出版历程
- 收稿日期: 2012-03-19
- 修回日期: 2012-04-27
- 刊出日期: 2012-05-30

Development of High Frequency Transformation of Indica Rice Mediated by Agrobacterium

XU Hui-bin^1,2,3,4,5,
WEI Yi-dong^1,2,3,4,5,
LIAN Ling^1,2,3,4,5,
HE Wei^1,2,3,4,5,
ZHANG Jian-fu^{1,2,3,4,5
, ,},
XIE Hua-an^{1,2,3,4,5
, ,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China;
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China;
3.
Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;
4.
National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China;
5.
South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian 350003, China

摘要

摘要: 系统分析影响根癌农杆菌介导籼稻遗传转化的主要因素,比较不同培养基、培养条件、继代次数、菌液浓度、侵染时间等因素对籼稻遗传转化的影响,并组建一个高效的籼稻遗传转化技术体系。结果表明,成熟胚或幼胚经过NMB培养基诱导,NB培养基继代培养这样的培养基组合比较适合籼稻胚性愈伤组织的培养。在农杆菌介导转化时控制好菌液浓度、侵染时间以及分化培养基中KT和NAA的浓度配比,并进行预分化处理,可显著提高抗性植株的分化效率,减少白化苗的产生,最终使转化率提高到8%。
- 根癌农杆菌 /
- 籼稻 /
- 遗传转化
Abstract: The major factors were analyzed and compared affecting the transformation frequency of indica rice mediated by Agrobacterium including medium, culture condition, subculture frequency, bacterial concentration and infection time and so on in the paper. At last a high transformation frequency of indica rice was set up. The results indicated that NMB medium for callus inducing and NB medium for callus subculturing were the best medium combination for tissue culture of indica rice. In the meanwhile, bacterial concentration, infection time and concentration ratio of KT and NAA in differential medium at the best level were modulated. The transformed calli were pre-differentiation. So the differentiation rate was improved significantly. The albino seedlings were reduced. Finally the transformation rate of indica rice was improved to 8%.
- Agrobacterium /
- indica rice /
- transformation