摘要:
以福建野生葡萄(VitisamurensisRupr.)带节茎段为外植体,建立无菌株系,并以此无菌株系小苗的茎段或叶片为外植体,进行愈伤组织诱导、继代增殖和长期保持等方面的研究。结果表明,光照条件下,愈伤组织诱导以MS+BA2.0mg·L-1+NAA0.2mg·L-1+蔗糖30g·L-1培养基最佳,诱导率可达100%;愈伤组织的继代增殖以MS+2,4-D1.0mg·L-1+蔗糖30g·L-1培养基最佳,可以获得生长状态良好的松散型愈伤组织;采用MS+2,4-D1.0mg·L-1+蔗糖30g·L-1培养基继代培养几代后转到MS+2,4-D1.0mg·L-1+KT0.5mg·L-1+AgNO35.0mg·L-1+蔗糖30g·L-1培养基上培养1代,如此交替进行,同时在光照条件下,可长期继代保持野生葡萄愈伤组织,并可保持其旺盛的生长能力。
关键词:
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葡萄 /
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外植体 /
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愈伤组织 /
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诱导 /
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继代保持
Abstract:
The plantlets of Fujian wild grapes (Vitis amurensis Rupr.) were established by using its stems with node as explants. And its callus induction, subculture and long-term maintenance were carried out by using the stems and leaves of these plantlets. The results showed that, with the illumination of lights, the effect of medium MS+BA 2.0 mg稬-1+NAA 0.2 mg稬-1+ Sucrose 30 g稬-1 revealed the best on inducing of calluses for a rate of 100%. The medium MS+2,4-D 1.0 mg稬-1+ Sucrose 30 g稬-1 was the best for the callus subculture multiplication to obtain the friable calluses in good growth. For a long-term maintenance, the calluses were sub-cultured for several generations with the medium MS+2,4-D 1.0 mg稬-1+ Sucrose 30 g稬-1 and then followed with the sub-culture of medium MS+2,4-D 1.0 mg稬-1+KT 0.5 mg稬-1+AgNO3 5.0 mg稬-1+Sucrose 30 g稬-1 for one generation. Continually, the calluses were alternately sub-cultured with the two mediums and could grow vigorously with the illumination of lights.
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