Abstract: Nitrogen (N) fertilizer management is one of the important factors in the rice cultural regulation, which plays a significant role in the growth of rice.Using the rice cultivar Yonyou No. 6 as experimental material, four different N application proportions at the early and late growth stages were carried out to understand the effects of N fertilizer management on rice yield and N utilization efficiency. In detail, the N application rates were the same for each treatment, but the N application proportions at the early and late growth stage were 4:6 (N1), 5:5 (N2), 6:4 (N3) and 7:3 (N4), respectively. The results indicated that appropriate N application rates at late growth stage, for example, N3 treatment (6:4), could effectively slow down the degradation of chlorophyll, reduce ineffective tillers and increase rice spike rate, which led to the high yield of rice. Moreover, the N accumulation, rice yield, agronomic and physiological nitrogen utilization rate in N3 treatment were more than other treatments. It's concluded that a optimized method of N fertilizer management could play an important role in the yield formation of Yonyou No. 6.