摘要:
为了评估和合理利用鲤种质资源,选出13个微卫星位点对框镜鲤、黑龙江鲤、荷包红鲤、兴国红鲤、黄河鲤和建鲤进行遗传多样性分析。结果显示:13个微卫星位点在6个鲤鱼群体中共检测出142个等位基因,所检测到的等位基因片段长度在116~280 bp。各鲤鱼群体的平均观察杂合度(Ho)、期望杂合度(He)分别在0.564~0.705和0.611~0.776;13个位点在6个鲤鱼群体中平均多态信息含量(PIC)在0.573~0.749。固定系数(FIS)分析表明,只有建鲤群体表现为杂合子过剩(平均FIS0)。试验结果表明,这6个鲤鱼群体多态信息含量丰富,遗传多样性水平较高,具有较大的选育潜力。群体间的遗传距离和聚类分析显示,框镜鲤与兴国红鲤亲缘关系最远,黄河鲤与建鲤的亲缘关系最近。
关键词:
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微卫星标记 /
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鲤 /
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群体 /
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遗传多样性
Abstract:
For evaluation and rational utilization of the germplasm resources of Common carp, the genetic diversity of Cyprinus carpio L, C.carpio haematopterus, C.carpio var.wuyuanensis, C.carpio var .singuonensis、C.carpio haematopterus Temminck et Schlegfl and C.carpio var.jian were analyzed with 13 microsatellite markers. In total, 142 alleles were detected in the six populations of common carp by the 13 microsatellite loci, whose DNA fragment length ranged from 116 bps to 280 bps. The average heterozygosity observed (Ho) varied from 0.564 to 0.705, and the heterozygosity expected (He) varied from 0.611 to 0.776 respectively in the six populations. The average polymorphic information content (PIC) of the 13 loci in the 6 populations of common carp ranged from 0.573 to 0.749. The average fixation index (FIS) showed heterozygote excess only in the C.carpio var. jian population,but heterozygote deficiency in the other 5 populations. The results suggested that rich polymorphism information content , large genetic diversity and great potential for selective breeding lied in the six populations. The genetic distance between each populations of the six Common carp populations and the UPGAM dendrogram showed that C.carpio L and C.carpio var .singuonensis were the farthest in relationship, and C.carpio haematopterus Temminck et Schlegfl and C.carpio var. jian were the nearest.
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