发酵产生5-氨基乙酰丙酸的科氏葡萄球菌的筛选

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项(2010R1025-4);福建省自然科学基金项目(2010J01106;福建省科技计划重大专项(2010NZ0002-3)

Screening of Staphylococcus cohnii for Bioproduction of 5-aminolevulinic Acid

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
School of Chemical Engineering, China University of Petroleum, Qingdao, Shandong266000, China;
3.
Agricultural Bio-resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要: 以发酵产生5-氨基乙酰丙酸(ALA)为目标,从自然界进行菌株的随机筛选,结果得到4株可生产5-氨基乙酰丙酸的细菌LA-2、LA-4、LA-7、LA-10。摇瓶发酵LA-10产量最高,达6.88 mg·L-1。在常规生理生化特性测定基础上,结合16S rDNA序列分析,LA-10鉴定为科氏葡萄球菌(Staphylococcus cohnii),命名为科氏葡萄球菌LA-10。
- 5-氨基乙酰丙酸 /
- 科氏葡萄球菌 /
- 筛选 /
- 发酵
Abstract: Four strains named LA-2, LA-4, LA-7 and LA-10 were distinguished by random screening of strains for bioproduction of 5-aminolevulinic acid.After shaking flask fermentation, the highest yield was 6.88 mg稬-1 from LA-10. The strain was identified as Staphylococcus cohnii based on the morphological, physiological , biochemical characteristics, and 16S rDNA analysis. It was named Staphylococcus cohnii LA-10.
- 5-aminolevulinic acid /
- Staphylococcus cohnii /
- screening /
- fermentation

[1]	SASIKALA C, RAMANA C V, RAO P R. 5-aminolevulinic Acid: A Potential Herbicide Insecticide from Microorganisms [J]. Biotechnology progress, 1994, 10(5):451-459.
[2]	MOAN J, PENG Q. An outline of the hundred-year history of PDT [J]. Anticancer research, 2003, 23(5A):3591.
[3]	FU W, LIN J, CEN P. Enhancement of 5-aminolevulinate production with recombinant Escherichia coli using batch and fed-batch culture system [J]. Bioresource Technology, 2008, 99(11):4864-4870.
[4]	张德咏, 刘勇, 成飞雪, 等. 一种高产5-氨基乙酰丙酸的工程菌. 中国专利:200610136870.7 ,2007-06-06.