Effect of lead stress on membrane and antioxidant systems in Vetiveria zizanioides leaves
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摘要: 以香根草为试验材料,研究不同浓度铅胁迫对叶片膜系统和抗氧化系统生理指标的影响.结果表明:当铅胁迫浓度低于500 mgL-1时,叶绿素含量、超氧化歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性较对照组高;铅浓度大于500mgL-1时,这些指标下降.随着铅浓度的增加,叶片细胞相对膜透性和丙二醛(MDA)的含量明显升高,AsA含量有极显著变化,而GSH变化不显著.当铅胁迫浓度在1000mgL-1以上时,香根草生长生物量极显著降低.Abstract: Effects of lead stress on the membrane and antioxidant systems in Vetiveria zizanioides leaves were studied.The results showed that the chlorophyll(Chl) content and SOD,POD and CAT activities increased,as compared to the control,when lead concentration was less than 500 mg L-1.These stress indices decreased when lead concentration was greater than 500 mg L-1.Meanwhile,the chlorophyll a/chlorophyll b ratio remained relatively constant.With increasing lead stress,the relative membrane permeability and MDA content increased considerably.A positive correlation between them was observed.The AsA content varied significantly,while GSH remained practically unchanged.And,the biomass of V.zizanioides decreased significantly at lead concentration of 1000 mgL-1 and 2000 mgL-1.
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Key words:
- lead stress /
- Vetiveria zizanioides /
- membrane system /
- antioxidant system
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[1] 陈玉成.污染环境生物修复工程[M].北京:化学工业出版社,2003:35-39. [2] 江行玉,赵可夫.植物重金属伤害及其抗性机理[J].应用与环境生物学报,2001,7:92-99. [3] 张宗申,利容千,王建波.外源Ca2+预处理对高温胁迫下辣椒叶片细胞膜透性和GSH、 AsA 含量及Ca2+分布的影响[J].植物生态学报,2001,25(2):230-234.
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