Determination of CoQ10 in Brown Rice of Different Genotypes
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摘要: 采用高效液相色谱法(HPLC)对糙米中辅酶Q10的含量进行测定,并对测定技术进行了优化.用该技术分析研究了100个水稻品种(含恢复系和种质资源)的辅酶Q10含量,其含量范围为0.30±0.03~5.36±0.04 mgkg-1,品种之间糙米辅酶Q10含量差异极显著.辅酶Q10含量最高的为恢复系4B233(5.36±0.04mgkg-1),是最低含量的178.67倍.Abstract: High performance liquid chromatography(HPLC)was employed to determine the CoQ10 in brown rice with different genotype.The testing methodology was optimized in the meantime.Content of CoQ10 in 100 varieties(including restore lines and germplasm resources of the rice)was determined by using HPLC.The contents ranged from 0.30?0.03 mgkg-1 to 5.36?0.04 mgkg-1 indicating very significant differences of CoQ10 content existing among the various brown rice varieties.The restore line 4B233 had the highest content of CoQ10(i.e.,5.36?0.04 mgkg-1),which was 178.67 times greater than the lowest.
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Key words:
- Brown rice /
- Coenzyme Q10 /
- HPLC
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[1] ZU Y G,ZHAO C J,LI C Y,et al.A rapid and sensitive LC-MS/MS method for determination of coenzyme Q10 in tobacco(Nicotiana tabacum L.)leaves[J].Journal of Separation Science,2006,29:1607-1612.
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