Abstract: Cultivar K326 for flue-cured tobacco was used to study the correlation between CCI and the chlorophyll content in the tobacco leaves.The result showed a significant positive correlation between the chlorophyll meter reading and the chlorophyll content.The correlation coefficients(R2) of CCI and chlorophyll content on different parts of a leaf were 0.8831 for the base,0.934 for the middle portion,and 0.8819 at the tip.Therefore,the middle part of a leaf would be the best choice for testing CCI for tobacco's chlorophyll content.This measurement method also had the advantages of being simple and convenient to use,as well as,non-intrusive to the sample.