铁皮石斛试管苗生根影响因素研究

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基金项目:

福建省教育厅科技项目(JA09099)

Factors affecting plantlet rooting from Dendrobium officinale tissue culture

1.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 以铁皮石斛试管丛生苗为原料,研究不同pH值、光照强度和温度对铁皮石斛苗长势和生根的影响。结果表明:温度和光照对铁皮石斛苗株高、生根率和苗鲜重影响显著(P0.05),而pH对铁皮石斛的苗株高影响不显著(P0.05),对苗鲜重和生根率影响显著;铁皮石斛丛生苗壮苗生根的适宜pH值为5.4,适宜温度为25℃,光照强度为1500lx。
- 铁皮石斛 /
- 组培 /
- 壮苗生根 /
- 影响因素
Abstract: Effects of pH,light intensity and temperature on the growth and root formation of Dendrobium officinale cultivated in vitro were studied.Results showed that the height,rate of root formation and fresh weight of D.officinale were affected significantly by light intensity and temperature(P0.05);and that the root formation and fresh weight were significant affected by pH(P0.05),but not on the plantlet height.The optimal conditions for the growth and root formation of D.officinale were: pH 5.4 at 25℃ with the light intensity of 1500 lx.
- Dendrobium officinale /
- tissue culture /
- growth /
- rooting

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