响应面法优化浒苔碱溶性多糖的提取工艺

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Optimization for akali-extraction of plysaccharides from Enteromorpha prolifera

摘要: 应用响应面分析法(RSM)优化浒苔碱溶性多糖的提取工艺。在单因素试验基础上,选取碱浓度、料液比、提取时间3个因素进行Box-Behnken中心组合设计,根据SAS系统回归分析得出浒苔碱溶性多糖的最佳工艺条件为:在100℃提取温度下,Na2CO3浓度0.85%、料液比1:60(m:v)、提取时间124min。在该条件下的响应面模型预测的多糖提取含量为13.42%。
- 浒苔 /
- 碱溶性多糖 /
- 提取 /
- 响应面分析
Abstract: Response surface methodology was applied to optimize the extraction of alkali-soluble polysaccharides from Enteromorpha prolifera.The factors investigated included alkali concentration,water/substrate ratio and extraction time.A three-level three-factor Box-Behnken design was applied for the experiment.The results showed that the optimum conditions for the alkali-extraction were: temperature at 100℃,alkali concentration of 0.85%,water/substrate ratio of 1/60,and extraction time of 124 min.The resultant extract contained 13.42% polysaccharides.
- Enteromorpha prolifera /
- alkali-soluble polysaccharides /
- extraction /
- response surface methodology

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