Diversity of Endophytic Bacteria in Root Nodules of Different Cultivars of Astragalus Sinicus L
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摘要:
目的 阐释紫云英根瘤内生菌的群落结构,为研发根瘤菌菌剂及了解固氮机制提供依据。 方法 以福建省种植的不同紫云英品种的根瘤为材料,借助Illumina Miseq PE300测序平台,以16S rRNA基因为靶标,研究不同紫云英品种的根瘤内生细菌的多样性。 结果 不同品种会影响紫云英根瘤内生细菌的类群和多样性。浙紫5号(ZZ5)的优势属为假单胞菌属(Pseudomonas),其余品种根瘤内生细菌的优势属均为中慢生根瘤菌属(Mesorhizobium)。闽紫8号(MZ8)、信阳(XY)和浙紫5号的根瘤内生细菌的多样性指数(Shannon)较高,除了共生固氮的中慢生根瘤菌外,还包括丰富的非根瘤菌,如假单胞菌属、寡养单胞菌属(Stenotrophomonas)、杜擀氏菌属(Duganella)、泛菌属(Pantoea)、欧文氏菌属(Erwinia)等。闽紫8号、信阳和浙紫5号这3个品种根瘤中含有较高丰度的致病菌大黄欧文氏菌(Erwinia rhapontici)和菠萝泛菌PA13( Pantoea ananatis PA13)。 结论 紫云英品种对根瘤内生细菌的群落结构有一定影响,浙紫5号的根瘤内生细菌主要为具促生作用的非根瘤菌假单胞菌,而其余品种为具固氮能力的根瘤菌,在生产实际中可以采用紫云英混种的方式达到固氮促生的双重功效。 Abstract: : [ Objective ] Analyzing the community structure of endophytic bacteria in root nodules is important to develop the rhizobium inoculants and understand the mechanism of fixing nitrogen. [ Method ] This study was conducted to investigate the diversity of endophytic bacteria in root nodules of A. sinicus. Seven cultivars of A. sinicus planted in Fujian province were selected to analyze the diversity of endophytic bacteria in root nodules using 16S rRNA gene as the target gene with Illumina Miseq PE300 sequencing platform. [ Result ] C ultivar could impact the composition of nodule endophytic bacteria. The most abundant genus in ZZ5 root nodule was Pseudomonas but the predominnat genus was Mesorhizobiumin in other cultivars. Furthermore, we found high Shannon indices of endophytic bacterial communities in the root nodules of MZ8, XY and ZZ5. These cultivars had a large diversity of non-rhizobial endophytes like Pseudomonas, Stenotrophomonas, Duganella and Erwinia, etc. The higher relative abundances of pathogen Erwinia rhapontici and Pantoea ananatis PA13 were observed in the root nodules of MZ8, XY and ZZ5. [ Conclusions ] The predominant endophytic bacteria of cultivar ZZ5 is non-rhizobial microorganism Pesudomonas which had the growth-promoting effect, while rhizobia are the predominant microorganism in other cultivars. Different cultivars can be applied in combination to get more effect.-
Key words:
- Astragalus sinicus /
- cultivar /
- root nodule /
- endophytic bacteria /
- diversity
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图 1 门水平不同紫云英品种根瘤内生细菌的相对丰度
MZ7:闽紫7号、MZ8:闽紫8号、GZ:光泽、XY:信阳、XZ:湘紫、YJZ:弋江籽、ZZ5:浙紫5号,下同。
Figure 1. Relative abundance of endophytic bacteria in root nodules of different cultivars of A. sinicus on phylum level
MZ7: Minzi No. 7, MZ8: Minzi No.8, GZ: Guangze; XY: Xinyang, XZ: Xiangzi, YJZ: Yijiangzi, ZZ5: Zhezi No. 5, the same below.
表 1 不同紫云英品种根瘤内生细菌的Alpha多样性指数
Table 1. Alpha diversity index of endophytic bacteria in root nodules of different cultivars of A. sinicus on genus level
样本
SampleAce指数
Ace indexChao1 指数
Chao1 indexShannon指数
Shannon indexSimpson指数
Simpson index覆盖度
Coverage/%闽紫7号(MZ7)
Minzi No. 7 (MZ7)26.26 24.25 0.07 0.98 99.98 闽紫7号(MZ8)
Minzi No.8 (MZ8)104.42 90.11 1.58 0.30 99.93 光泽(GZ)
Guangze (GZ)25.86 23.67 0.08 0.98 99.98 信阳(XY)
Xinyang (XY)191.81 191.50 1.73 0.40 99.92 湘紫(XZ)
Xiangzi (XZ)97.85 68.60 0.14 0.96 99.94 弋江紫(YJZ)
Yijiangzi (YJZ)23.60 23.00 0.11 0.96 99.98 浙紫5号(ZZ5)
Zhezi No.5 (ZZ5)72.45 84.50 1.13 0.53 99.95 -
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