激素水平对福建道地瓜蒌组培快繁的影响

郑向丽; 叶花兰; 徐国忠; 陈敏健

激素水平对福建道地瓜蒌组培快繁的影响

郑向丽^1,2,
叶花兰^1,2,
徐国忠^1,2,
陈敏健^1,2, ,

1.
福建省农业科学院农业生态研究所,福建福州 350013;
2.
福建省丘陵地区循环农业工程技术研究中心,福建福州 350013

基金项目:

福建省农业科学院科技创新团队建设基金项目(STIF-Y01);福建省农业科学院双百行动计划项目(sbmb1104-1);福州市科技计划项目(2011-S-76)

详细信息

作者简介:
郑向丽(1978-),女,硕士,助理研究员,主要从事植物组培及牧草育种研究([BF]E-mail:hhuadi@163.com)

通讯作者:
陈敏健(1957-),男,副研究员,主要从事规范化种植和农业生态循环应用研究(E-mail:cnj038@126.com)

中图分类号: S 567
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出版历程
- 收稿日期: 2011-07-07
- 修回日期: 2011-09-06
- 刊出日期: 2011-10-15

Effect of Hormone on Tissue Culture and Rapid Propagation of Trichosanthes kirilowii Maxim

1.
Fujian Engineering and Technology Research Center for Hilly Prataculture, Fuzhou, Fujian 350013, China;
2.
Fujian Engineering and Technology Research Center for Recycling Agriculture, Fuzhou, Fujian 350013, China

摘要

摘要: 以福建道地瓜蒌带腋芽的茎段为外植体,MS为基础培养基,研究不同激素水平对福建道地瓜蒌组织培养的影响,探讨福建道地瓜蒌快速繁殖的有效途径。结果表明:瓜蒌带腋芽的茎段接种于MS+1.0 mgL-16-BA 蔗糖培养基上,15 d后外植体出现许多绿色的小芽点,芽点健壮;30 d后,诱导出的丛生芽数平均达6.5个,长势快,平均出芽率达81.25%。分离正常的幼芽在MS+0.4 mgL-1NAA的生根培养基上,效果最好,15 d后长出不定根,再生成完整的瓜蒌植株,平均生根5条,根系粗壮、发达,生根整齐,生根率达100%。通过瓜蒌带腋芽茎段的组织培养,可以对瓜蒌种苗进行脱毒和品种更新复壮,解决瓜蒌以种子繁育雌雄比例不协调的问题,可在短期内大量繁殖瓜蒌雌雄种苗,具有成本低,效益高的特点。
- 福建 /
- 瓜蒌 /
- 组织培养 /
- 激素水平
Abstract: Effect of hormone application on tissue culture of Trichosanthes kirilowii Maxim was investigated. Stems with axillary buds were used as explantlet,and MS for the basic medium. A vailable methods for the rapid propagation to clone T. kirilowii Maxim are reviewed.In the experiment, the young adventitious buds continuously appeared for 15 days in the culture medium of MS + 1.0 mgL-1 BA. In 30 days, the average number of shoots was 6.5, and the average shoot ratio was 81.25%. The excised plantlets could produce root 15 days after inoculation on the MS + 0.4 mg NAA L-1 medium. They continued to gradually develop into normal plants. A single plant had an average of 5 roots. The average rooting percentage was 100%. This rapid propagation method for T. kirilowii Maxim appeared to offer many benefits at low cost.
- Fujian /
- Trichosanthes kirilowii Maxim /
- tissue culture /
- hormone application

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参考文献(5)

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