摘要:
应用GC-MS对10种不同初始pH条件下发酵的FJAT-0809-GLX发酵液的丙酮萃取液进行初步成分鉴定,从这10种发酵液中得到匹配率≥90%成分11种,各发酵液中的成分存在明显差异,其中共有成分1种,为六氢吡咯并吡嗪-1,4-二酮。在匹配率≥90%成分中,功能性成分按其功能可分为2类,一类为挥发性或芳香类物质,与该发酵液具有的特殊香气有关,包括5-甲基呋喃醛、2-甲基-3-羟基-4-吡喃酮、苯乙醛、棕榈酸、顺式十八碳-9-烯酸、甲基环戊烯醇酮;另一类为防腐或抑菌类成分,包括2-甲基-3-羟基-4-吡喃酮、2-呋喃甲醇、六氢-吡咯吡嗪-1, 4-二酮和5-羟甲基糠醛。初始pH 6~12的发酵液对大肠杆菌K88均有抑制效果,其中初始pH为7的抑菌效果最佳,抑菌圈直径达到20.33 mm。不同的初始pH影响着发酵终点pH,进而影响着短短芽孢杆菌FJAT-0809-GLX次生代谢物的产生。
Abstract:
Components of FJAT-0809-GLX fermentation liquid in10 different initial pH values were analyzed by GC-MS. Eleven compounds with the match degree above 90% were identified. The compounds found commonly in these 10 fermentation liquid was Hexahydro-pyrrolo pyrazine-1,4-dione.There were two sorts of functional compounds in the FJAT-0809-GLX fermentation liquids; one is volatile compounds which relate to the special aroma of this fermentation liquid, including 5-methyl-2-Furancarboxaldehyde,Maltol,Benzeneacetaldehyde, n-Hexa-decanoic acid, Oleic Acid, and 2-Hydroxy-3-methyl-2-cyclopenten-1-one. The other sort of functional compound had antiseptic and bacteriostatic effect, including Maltol, 2-Furanmethanol and Hexahydro-pyrrolo pyrazine-1,4-dione and 5-(hydroxymethyl)-2-Furancarboxaldehyde. The fermentation liquid with the initial pH value of 6 to 12 had inhibitory activity against K88.The pH value 7 had the best inhibitory activity; the diameter of inhibition zone was 20.33mm. Different initial pH values led to different terminal pH value, and then affect the generation properties of secondary metabolites of Brevibacillus brevis FJAT-0809-GLX.
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