脂肪酸甲酯谱图分析方法及其在微生物学领域的应用

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名

邮箱

手机号码

标题

留言内容

验证码

基金项目:

国家863计划项目(2006AA10A211);福建省发展和改革委员会重点项目[闽农产(2006)10号];福建省青年科技人才创新项目(2003.J046)

Fingerprint analysis on methyl fatty acid and its applications in microbial study

1.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China

摘要: 脂肪酸甲酯谱图分析方法(Fatty Acid Methyl Ester,FAME)是鉴于脂肪酸可作为生物标记物而发展起来的分析技术。本文介绍了FAME谱图分析方法及其在微生物学领域的应用,包括在微生物检测、鉴定和微生物多样性研究中的应用。
- FAME谱图分析 /
- GC/GCMS /
- 微生物鉴定 /
- 微生物多样性
Abstract: Fatty acid is an important biomarker.Its application in biology research has become increasingly popular.The method of FAME fingerprint analysis is based on fatty acid’s utilization as a biomarker.This paper describes the FAME fingerprint analysis and its applications for microbial studies,including identification of unknown microbes and assessment of microbial diversity.
- FAME fingerprint analysis /
- GC/GC-MS /
- microbial identification /
- microbial diversity

[1]	杜宗敏,杨瑞馥.生物标记物在微生物鉴定和检测中的应用[J].微生物学免疫学进展,2003,31(3):67-73.
[2]	周德庆.微生物学教程[M].北京:高等教育出版社,2002.
[3]	VANDAMME P,POT B,GILLIS M,et al.Polyphasic taxonomy,a consensus approach to bacterial systematics[J].Microb Rev,1996,60:407-438.
[4]	BASILE F,VOORHCES K J,HADTLELD T L.Microorganism gram-type differentiation based on pyrolysis-mass spectrometry of bacterial fatty acid methyl ester extracts[J].Appl Environ Microbiol,1995,61(4):1534-1539.
[5]	WISELY S M,MCDONALD D B,BUSKIRK S W.Evaluation of the genetic management of the endangered black-footed ferret (Mustela nigripes)[J].Zoo Biology,2003,22:287 298.
[6]	KIDD S,GARCHOW H,ODELSON D A,et al.Accuracy,reproducibility,and interpretation of fatty acid methyl ester profiles of model bacterial communities[J].Appl Environ Microbiol,1994,60(7):2483-2493.
[7]	RITCHIE N J.Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil[J].Applied and Environmental Microbiology,2000,1668-1675.

点击查看大图