艾GRF基因家族的鉴定与表达分析

郭晨宁; 李晶晶; 杨晶凡; 刘俊; 杨灏; 马蕊; 陈随清; 练从龙

doi:10.19303/j.issn.1008-0384.2024.05.005

艾GRF基因家族的鉴定与表达分析

doi: 10.19303/j.issn.1008-0384.2024.05.005

郭晨宁^{1, 2, 3,},
李晶晶^{1, 2, 3},
杨晶凡^{1, 2, 3},
刘俊^{1, 2, 3},
杨灏^{1, 2, 3},
马蕊^{1, 2, 3},
陈随清^{1, 2, 3},
练从龙^{1, 2, 3, ,}

1.
河南中医药大学药学院，河南　郑州　450046
2.
河南省中药资源与中药化学重点实验室，河南　郑州　450046
3.
豫药全产业链研发河南省协同创新中心，河南　郑州　450046

基金项目: 河南省高等学校重点科研项目（22A360012）；河南省中药材产业技术体系建设专项（豫科项〔2022〕30号）；国家自然科学基金项目（82204551）

详细信息

作者简介:
郭晨宁（1999 —），女，硕士研究生，主要从事药用植物分子生物学研究，E-mail：gcning6850@163.com

通讯作者:
练从龙（1988 —），男，博士，讲师，主要从事药用植物生理生态与分子生物学研究，E-mail：lian1988@hactcm.edu.cn

中图分类号: R286
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- 被引次数: 0
出版历程
- 收稿日期: 2024-03-19
- 修回日期: 2024-04-16
- 网络出版日期: 2024-06-26
- 刊出日期: 2024-05-28

Identification and Expressions of GRF Genes in Artemisia argyi

GUO Chenning^{1, 2, 3
,},
LI Jingjing^{1, 2, 3},
YANG Jingfan^{1, 2, 3},
LIU Jun^{1, 2, 3},
YANG Hao^{1, 2, 3},
MA Rui^{1, 2, 3},
CHEN Suiqing^{1, 2, 3},
LIAN Conglong^{1, 2, 3
, ,}

1.
School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan　450046, China
2.
Henan Key laboratory of Resource and Chemistry for Chinese Traditional Medicine, Zhengzhou, Henan　450046, China
3.
Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Zhengzhou, Henan　450046, China

摘要

摘要: 目的生长调节因子（growth-regulating factors, GRF）是植物的一种特有蛋白，探明艾（Artemisia argyi）GRF基因家族的生物学特性，为艾的生长发育和逆境胁迫调节提供依据。方法对艾GRF基因家族进行理化性质、进化、基因结构以及表达等生物信息学进行分析，进而通过转录组数据和实时荧光定量PCR（qRT-PCR）技术分析基因家族各成员在不同组织、逆境胁迫以及响应不同激素的表达模式。结果从艾基因组中共鉴定出17个GRF家族成员，理化鉴别显示其编码蛋白均为亲水蛋白；系统发育进化树分为4个亚家族；motif分析表明进化树中同一分支的基因具有相似或相同的保守基序；AaGRF基因家族成员随机分布在11条不同的染色体上；AaGRF基因家族启动子含有多种逆境和激素响应元件；AaGRF基因家族在响应盐碱胁迫时表现出不同的表达趋势；该家族成员在艾的根、茎、叶中的表达模式具有组织特异性；经脱落酸、吲哚乙酸、水杨酸和茉莉酸甲酯处理后，AaGRF05、AaGRF06、AaGRF11和AaGRF14在处理后的12 h基因表达量最高，表明大部分基因在前期参与不同激素的调控。结论艾GRF基因家族参与艾生长发育、激素与逆境胁迫调节，为探究GRF家族功能提供的理论依据。
- 艾 /
- GRF基因家族 /
- 表达分析 /
- 激素响应
Abstract: Objective Growth-regulating factor (GRF) genes in Artemisia argyi were identified and analyzed. Method Bioinformatic analysis on the physiochemical properties, structure, and evolution of the GRF genes, as well as their expressions in different tissues under stresses, of A. argyi was conducted using qRT-PCR. Result SeventeenAsGRFs were hydrophilic proteins in the phylogenetic evolutionary tree of 4 subfamilies. Those genes of same clade in the evolutionary tree contained a similar or same conserved motif. The members were randomly distributed on 11 chromosomes with promoters of multiple stress- and hormone-responsive elements and one that varied in the response to salinity. The tissue-specific expressions of the genes, including AaGRF05, AaGRF06, AaGRF11, andAaGRF14, peaked in 12h after being exposed to abscisic acid, indoacetic acid, salicylic acid, or methyl jasmonate. Conclusion The GRF genes associated with the growth and stress regulation of A. argyi were identified, and relevant bioinformatics obtained.
- Artemisia argyi /
- GRF gene family /
- expression analysis /
- hormone response

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图 1 AaGRF家族系统进化树

AT：拟南芥；Gh：棉花；Ha：向日葵；Aa：艾。

Figure 1. Phylogenetic tree of AaGRFs

AT: Arabidopsis thaliana; Gh: Gossypium hirsutum; Ha: Helianthus annuus; Aa: A. argyi.

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图 2 艾GRF基因家族的结构、结构域和motif

Figure 2. Structures, domains, and motifs of AaGRFs

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图 3 拟南芥GRF家族基因组结构、保守结构域和motif分析

Figure 3. Structures, conserved domains, and motifs of GRFs in A. thaliana

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图 4 AaGRF基因家族在染色体上的定位

Figure 4. Mapping of AaGRFs chromosomes

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图 5 AaGRF基因家族顺式作用元件

Figure 5. Cis-acting elements in AaGRFs

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图 6 AaGRF基因家族响应盐和盐碱胁迫的表达模式

CK_L：空白对照组叶片；SAT_L：盐碱处理组叶片；ST_L：盐处理组叶片；CK_R：空白对照组根；SAT_R：盐碱处理组根；ST_R：盐处理组根。

Figure 6. Expressions of AaGRFs in responses to salt- and saline-alkali-stresses

CK_L：Blank control group leaves；SAT_L：Salt alkali treatment group leaves；ST_L：Salt treatment group leaves；CK_R：Blank control group root；SAT_R：Salt alkali treatment group root；ST_R：Salt treatment group root.

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图 7 AaGRF家族成员在艾的根、茎、叶中的表达模式

不同小写字母代表在 0.05 水平上差异显著。图8同。

Figure 7. Expressions of AaGRFs in roots, stems, and leaves of A. argyi

Data with different lowercase letters represent significant differences at P＜0.05. Same for Fig. 8.

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图 8 AaGRF家族成员响应激素处理的表达模式

Figure 8. Expressions of AaGRFs in response to hormone treatment

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表 1 AaGRF基因家族蛋白理化性质

Table 1. Physiochemical properties of AaGRF proteins

基因名称 Gene name	基因组ID Genome ID	长度 Length/aa	分子量 Molecular weight /Da	等电点 Isoelectric point(pI)	不稳定指数 Instability index	脂肪族指数 Aliphatic index	疏水指数 Hydrophobic index
AaGRF01	Aarg02G031430.1	350	39886.41	8.35	51.62	50.43	−0.921
AaGRF02	Aarg04G019450.1	471	50852.5	9.07	41.98	64.86	−0.547
AaGRF03	Aarg05G013120.1	315	36666.98	7.26	71.34	58.83	−0.9
AaGRF04	Aarg06G029080.1	364	40414.66	8.87	48.27	52.77	−0.91
AaGRF05	Aarg06G030040.1	394	43558.38	9	48.32	57.92	−0.717
AaGRF06	Aarg06G030730.1	380	42917.36	8.13	56.92	54.92	−0.874
AaGRF07	Aarg08G013810.1	138	15560.65	7.66	52.91	65.72	−0.541
AaGRF08	Aarg08G014980.1	339	38274.79	8.02	49.95	43.42	−0.871
AaGRF09	Aarg10G061780.1	335	38176.52	8.79	49.88	52.39	−0.87
AaGRF10	Aarg11G000710.1	329	37157.12	8.97	47	52.52	−0.829
AaGRF11	Aarg11G015240.1	501	54688.51	7.24	42.86	59.38	−0.635
AaGRF12	Aarg12G018140.1	472	51001.69	9.16	42.64	64.51	−0.56
AaGRF13	Aarg14G027820.1	364	40379.65	8.72	45.86	51.43	−0.898
AaGRF14	Aarg14G028850.1	352	38751.75	8.44	51.96	54.29	−0.709
AaGRF15	Aarg16G013730.1	138	15512.59	7.66	52.9	69.28	−0.509
AaGRF16	Aarg16G015010.1	339	38280.78	7.66	49.46	45.13	−0.853
AaGRF17	Aarg17G035750.1	382	41725.01	6.67	45.33	56.18	−0.678

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