TGEV与PEDV核衣壳蛋白双价基因原核质粒的构建与表达

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名

邮箱

手机号码

标题

留言内容

验证码

基金项目:

福建省财政专项(STIF-Y02);福建省属公益类科研院所基本科研专项(2010R1101003-1)

Prokaryotic expression plasmid with nucleocapsid genes of TGEV/PEDV and co-expression of two genes

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350013, China;
2.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China;
3.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 运用DNA重组技术将猪传染性胃肠炎病毒核衣壳基因、猪流行性腹泻病毒核衣壳基因编码最大局部亲水性抗原决定簇基因片段进行串联,克隆到原核表达载体pET-32a(+)中,构建预防仔猪冠状病毒性腹泻双价基因原核表达质粒。将该质粒转化至大肠杆菌BL21(DE3)中,经IPTG诱导后,SDS-PAGE电泳结果显示,双价重组基因表达出约86.8 kD的融合蛋白。Western-blotting检测结果表明,该融合蛋白具有良好的反应原性。
- 猪传染性胃肠炎病毒 /
- 猪流行性腹泻病毒 /
- 核衣壳蛋白 /
- 原核表达 /
- 共表达
Abstract: By using DNA recombination technology,nucleocapsid gene from swine transmissible gastroenteritis virus was lined with the gene fragment of the maximum local hydrophilic antigenic determinant of nucleocapsid gene from porcine epidemic diarrhea virus.The recombinant gene was cloned into the prokaryotic expression vector pET-32a(+).Then,the prokaryotic expression plasmid with the bivalent gene to prevent piglet’s coronaviral diarrhea was constructed.After transforming the plasmid into E.coil BL21(DE3),the bacteria were induced by IPTG with SDS-PAGE analysis showing the bivalent gene expressed efficiently,as well as,the western-blot test expressing the fusion protein.
- swine transmissible gastroenteritis virus /
- porcine epidemic diarrhea virus /
- nucleocapsid protein /
- prokaryotic expression /
- co-expression