福建省蛋鸡血管瘤型J亚群白血病的分子生物学诊断

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Microbiological diagnosis of ALV-J from hemangioma layer in Fujian

1.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要: 针对福建省某鸡场的蛋鸡群发生产蛋下降和部分死亡,病鸡脚趾明显呈现血管瘤特征,运用实时荧光定量PCR方法对病鸡组织和血液样品进行检测,检出了J亚群白血病病毒(ALV-J);通过基因序列的比较分析,发现其氨基酸序列与国外及国内其他省份报导的ALV-J有很高的同源性,从病原分子生物学诊断角度证实福建省蛋鸡群中ALV-J的存在。
- 蛋鸡 /
- 血管瘤 /
- J亚群禽白血病 /
- 荧光定量PCR
Abstract: Layers from one of the chicken houses in Fujian showed egg laying reduction and some died.Hemangioma was observed on their claws.Using a real-time florescence quantitative polymerase chain reaction method,ALV-J was found in the tissue and blood samples of the infected layers.The sequence analysis further indicated that the viruses were highly homologous to those reported previously at home and abroad.This was the first time the existence of ALV-J in domestic layers in Fujian was confirmed by means of a pathogenic microbiological diagnostic method.
- layer /
- hemangioma /
- ALV-J /
- real-time florescence quantitative polymerase chain reaction

[1]	XU B R,DONG W X,YU C M,et al.Occurrence of avian leukosis virus subgroup J in commercial layer flocks in China[J].Avian Pathology,2004,33 (1):13-17.
[2]	ZHANG X,LIAO M,JIAO P,et al.Development of aloopmediated isothermal amplification assay for rapid detection of subgroup J avian leukosis virus[J].J Clin Microbiol,2010,48(6):2116-2121.

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