高效液相色谱法同时测定鱼油中维生素A、D和E

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Simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography

1.
Central Laboratory, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要: 试验采用高效液相色谱法同时测定鱼油中维生素A、D和E,以保留时间和特征光谱图进行定性,以紫外最强吸收波长的色谱图的峰面积进行定量分析。结果表明,该方法回收率为89.6%～95.0%,相对标准偏差为3.6%～5.1%,可适用于鱼油中维生素A、D和E的测定。
- 高效液相色谱(HPLC) /
- 维生素 /
- 鱼油
Abstract: A method for the simultaneous determination of vitamin A, D and E in fish oil by high performance liquid chromatography was developed.The quantitative analysis on the vitamin content in fish oil was made by characterizing the retention time and spectral graph, and using the maximum absorbance wavelength for peak area.The results showed that the average recovery was 89.6%—95.0%,and variety was 3.6%—5.1%.So this method was suitable to be used in the determination on vitamin A, D and E.
- High performance liquid chromatography /
- Vitamin /
- Fish oil

[1]	Sprecher H,James A T.Biosynthesis of long chain fatty acids in mammalian systems[A].Emken EA,Dutton HJ,edi tor.Geometrical and Positional Fatty Acid Isomers[M].Champaign,IL:The American Oil Chemists' Society,1979.303-338.
[2]	骆华星,周德权.鱼油抗氧化剂的筛选[J].预防医学文献信息,2000,6(1):21-22.
[3]	GB/T 5009.82-2003,食品中维生素A和维生素E的测定[S].

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