大豆防御应答相关<i>WRKY</i>转录因子的克隆与表达分析

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大豆防御应答相关WRKY转录因子的克隆与表达分析

基金项目:

福建省青年科技人才创新项目(2006F3026);农业部公益性行业(农业)科研专项(200903002);福建省属公益类科研院所基本科研专项(2009R10027-3)

1.
Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Key Laboratory of Crop Germplasm Utilization between Fujian and Taiwan, Ministry of Agriculture Fuzhou, Fujian 350013, China;
3.
Fujian Provincial seed station, Fuzhou, Fujian 350003, china

摘要: WRKY转录因子与植物的生物和非生物胁迫应答密切相关。通过RT-PCR方法获得了与拟南芥转录因子WRKY14的相似性高达92%的大豆WRKY转录因子GmWRKY的cDNA序列,并利用半定量RT-PCR分析了GmWRKY在大豆不同组织中及不同非生物胁迫下的表达情况。结果表明:GmWRKY在大豆根、茎、叶中均有表达。在铝、低磷、盐等胁迫下,GmWRKY表达均有不同程度的增加,说明非生物胁迫下GmWRKY在大豆抗逆中可能发挥重要作用。
- 大豆 /
- WRKY /
- 半定量反转录-聚合酶链反应 /
- 非生物胁迫
Abstract: WRKY transcription factor family is one of its large kinds that involve in the biotic and abiotic responses.GmWRKY cDNA sequence,which had a 92% homology with Arabidopsis thaliana transcription factor WRKY14,was obtained by RT-PCR.Its expression pattern in different tissues and under abiotic stresses was analysed by semi-quantitative RT-PCR.The result showed that GmWRKY expressed in soybean roots,stems,as well as,leaves,and the expression was strongly induced by abiotic stresses,such as aluminum,P deficiency and salt.It suggested that GmWRKY could play an important role in stress resistance of soybean under abiotic stresses.
- soybean /
- WRKY /
- semi-quantitive RT-PCR /
- abiotic stresses