油菜种子老化处理后的生理生化变化

顾炳朝; 岳绪国; 杨军; 唐泽庆

doi:10.19303/j.issn.1008-0384.2013.04.008

油菜种子老化处理后的生理生化变化

doi: 10.19303/j.issn.1008-0384.2013.04.008

江苏丘陵地区镇江农业科学研究所,江苏句容,212400

基金项目:

江苏省农业科技自主创新资金项目[cx(11)1026]

江苏省科技支撑计划(BE2012327)

详细信息

作者简介:
顾炳朝(1957-),男,副研究员,主要从事油菜育种与品种推广工作(E-mail:gbc0115@163.com)

中图分类号: S565.4
计量
- 文章访问数: 256
- HTML全文浏览量: 44
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2013-02-18
- 刊出日期: 2013-04-18

Physiological and Biochemical Characteristics of Artificially Aged Rapeseeds

Zhenjiang Agricultural Research Institute,Jurong,Jiangsu 212400,China

摘要

摘要: 以油菜品种‘镇油6号′种子为材料,研究油菜种子在高温(50℃)高湿(相对湿度100%)老化处理后的萌发特性、种子浸出液相对电导率、丙二醛、可溶性蛋白和可溶性糖含量以及抗氧化酶活性的变化。结果表明,随着老化时间的增加,油菜种子的发芽势、发芽率、发芽指数及其活力指数均显著降低。同时,随着老化天数的延长,浸出液电导率显著性增大,浸出液可溶性糖含量则逐渐升高;可溶性蛋白含量表现出下降趋势;种子MDA含量呈现先升高后逐渐下降的趋势。此外,在油菜种子老化进程中,SOD、POD、CAT活性变化的趋势基本一致,其活性均随老化天数的延长而逐渐降低;研究发现,在油菜种子老化处理中,种子浸出液电导率、可溶性蛋白含量和可溶性糖含量与种子老化及劣变程度显著性相关;膜脂过氧化作用也可能是引起或加剧种子老化劣变的重要原因之一。
- 油菜 /
- 种子老化 /
- 人工劣变
Abstract: Using seeds of Zhenyou No.6,we studied the germination characteristics,relative electric conductivity,MDA content,soluble protein and soluble sugar,as well as the antioxidant-related indexes of the seeds in response to the artificial aging at 50℃ and RH 100%.The results showed that(a)the seed′s moisture content increased considerably in the course of the aging;(b)the rate,energy,index and vigor of the seed germination decreased with aging;(c)the relative electrolyte leakage and soluble sugar content in the seeds increased,while the soluble protein decreased;(d)the seed′s MDA content increased initially and followed by a decline;and(e)upon aging,SOD,POD and CAT activities in the seeds decreased significantly.In conclusion,during aging,the relative electrolyte leakage,soluble protein and soluble sugar contents of the seeds positively correlated with the its vigor index.It was speculated that the deterioration of the rapeseeds as they aged was induced by the increased peroxidation of the membrance lipids.
- Rapeseed /
- artificial aging /
- seed deterioration

HTML全文

参考文献(20)

[1]	毛培胜,常淑娟,王玉红,等.人工老化处理对羊草种子膜透性的影响[J].草业学报,2008,17(6):66-70.
[2]	KALPANA R,MADHAVA R K V.Protein metabolism ofseeds of pigeonpea(Cajanus cajan L.Millsp)cultivars duringaccelerated ageing[J].Seed Science and Technology,1997,25:271-279.
[3]	BEGNAMI C N,CORTELAZZO A L.Cellular alterationsduring accelerated aging of French bean seeds[J].Seed Scienceand Technology,1997,24:295-303.
[4]	THAPLIYAL R C,CONNOR K F.Eeffects of acceleratedageing on viability,leachate exudation,and fatty acid content ofDalbergia sisso Roxb[J].Seed Science and Technology,1997,25:311-319.
[5]	AUNG U T,MCDONALD M B.Changes in esterase activityassociated with peanut(Arachis hypogaea L.)seeddeterioration[J].Seed Science and Technology,1995,23:101-111.
[6]	KALPANA R,MADHAVA R K V.Nucleic acid metabolismof seeds of pigeonpea[Cajanus cajan(L.)Millsp]cultivarsduring accelerated ageing[J].Seed Science and Technology,1997,25:293-301.
[7]	孙群,王建华,孙宝启.种子活力的生理和遗传机理研究进展[J].中国农业科学,2007,40(1):48-53.
[8]	LEHNER A,MAMADOU N,POELS P.Changes in solublecarbohydrates,lipid peroxidation and antioxidant enzymeactivities in the embryo during aging in wheat grains[J].Journal of Cereal Science,2008,47:555-565.
[9]	颜启传.种子学[M].北京:中国农业出版社,2001.
[10]	国际种子检验协会.1996国际种子检验规程[S].北京:中国农业出版社,1996:187-188.
[11]	宋松泉,傅家瑞.黄皮种子脱水敏感性与脂质过氧化作用[J].植物生理学报,1997,23(2):163-168.
[12]	KUMAR G N M,KNOWLRS N R.Changes in lipidperoxidation and lipolytic and free radical scavenging enzymeactivities during aging and sprouting of potato(Solanumtuberosum)seed tubers[J].Plant Physiol,1993,102:115-124.
[13]	ARORA R,WISNIEWSKI M E.Cold acclimation ingeneticacally related(sibling)deciduous and evergreen peach:A 60-k Dabark protein in cold acclimated tissue of peach is heatstable and related to the dehydrin family of proteins[J].PlantPhysiol,1993,105:95-101.
[14]	李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[15]	DE FORCHETTI S M,TIGIER H A.Eeffect ofdicarboxylicacids on the peroxidase-IAA oxidase isozymes ofsoybean callus[J].Physiol Plant,1983,59:355-358.
[16]	KALIR A,POLIJAKOFF A.Changes in activity of malatedehydrogenase,catalase,peroxidase and superoxide dismutasein leaves of Halimione portulacoides(L)Aellen exposed tohigh sodium chloride concentrations[J].Ann.Bot.,1981,47:75-78.
[17]	CHING T M,SCHOOLCRAFT I.Physiological and chemicaldifferences in aged seed[J].Crop Science,1968,(8):407-409.
[18]	毛培胜,韩建国.贮藏处理对老芒麦种子活力的影响[J].草业科学,2003,(4):16-19.
[19]	刘宣雨,刘树君,程红焱,等.甜高粱种子人工老化过程中活性氧消除酶活性的变化[J].植物生理学通讯,2008,44(4):719-721.
[20]	薄丽萍,吴震,蒋芳玲,等.不结球白菜种子活力及抗氧化特性在人工老化过程中的变化[J].西北植物学报,2011,31(4):0724-0730.