Development and Application of Ligase-dependent RT-PCR Assay for Detection of Avian Tembusu Virus
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摘要: 根据连接酶依赖PCR工作原理设计并建立一种禽坦布苏病毒RT-PCR快速检测方法。结果显示, 该方法能特异性检测不同禽源的坦布苏病毒分离株, 且与常规RT-PCR检测敏感性相当。运用该方法对动物攻毒试验采集的6份样品及20112012年间采集自福建、江西、浙江及广东省发病鸭场的140份样品进行检测。动物试验样品均为阳性, 临床样品检出率为14.3% (20/140) 。试验结果表明, 本试验所建立的连接酶依赖RT-PCR方法具有快速、敏感、特异等优点, 可用于禽坦布苏病毒的流行病学调查及病毒检测。
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关键词:
- 禽坦布苏病毒 /
- 连接酶依赖RT-PCR /
- 病源检测
Abstract: Based on the mechanism of ligase-dependent RT-PCR assay, we established RT-PCR assay to detect avian tembusu virus which still prevails in most poultry of China.The results showed that it would specifically detect tembusu viruses isolated from different avian, such as laying duck, meat duck, goose and hens.The sensitivity of this assay is equivalent to that of conventional RT-PCR.To test the reliability of ligase-dependent RT-PCR assay, six tissue samples collected from experimental animals and 140clinical samples collected from Fujian, Jiangxi, Zhejiang, and Guangdong province during 2011-2012were detected.All of the six tissue samples were positive, while only 20of the 140clinical samples were tested to be positive by this method.The above results indicated that the ligase-dependent RT-PCR assay was a sensitive, specific and rapid diagnosis method for detecting avian Tembusu virus.-
Key words:
- avian tembusu virus /
- ligase-dependent RT-PCR assay /
- pathogen detection
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