禽坦布苏病毒NS1基因的原核表达

潘异哲; 万春和; 黄瑜; 程龙飞; 傅光华; 施少华; 陈红梅

doi:10.19303/j.issn.1008-0384.2013.07.005

禽坦布苏病毒NS1基因的原核表达

doi: 10.19303/j.issn.1008-0384.2013.07.005

1. 福建省农业科学院畜牧兽医研究所,福建福州 350013;
2. 江西农业大学动物科学技术学院,江西南昌 330045

基金项目:

福建省自然科学基金项目 (2012J01112)

福建省种业创新与产业化工程项目 (FJZYCX-9)

现代农业产业技术体系建设专项 (CARS-43)

福建省财政专项——福建省农业科学院科技创新团队重点建设项目 (STIF-Y02)

详细信息

作者简介:
潘异哲 (1986-), 男, 硕士研究生, 主要从事水禽病毒分子生物学研究 (E-mail:ikey_zz@qq.com);万春和 (1982-), 男, 助理研究员, 主要从事水禽病毒分子生物学研究 (E-mail:chunhewan@126.com)

中图分类号: S852.65
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出版历程
- 收稿日期: 2013-02-03
- 刊出日期: 2013-07-18

Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Science;
2. College of Animal Science and Technology,Jiangxi Agricultural University

摘要

摘要: 根据GenBank中登录的禽坦布苏病毒 (avian Tembusu virus, ATMV) WR株非结构蛋白NS1基因设计特异性引物, 采用RT-PCR方法从禽坦布苏病毒WR株核酸中扩增其NS1基因片段, 并将其克隆到原核表达载体pGEX-4T-1上构建原核表达重组质粒pGEX4T-NS1, 进行条件优化诱导表达, 将表达的目的蛋白经SDSPAGE分析。结果表明, 分子量约为62kD的重组目的蛋白得到表达。
- 禽坦布苏病毒 /
- NS1基因 /
- 原核表达
Abstract: A pair of primers were designed and synthesized according to the nanostructure protein (NS1) gene of avian Tenbusu virus WR strain available in GenBank.The NS1gene was amplified by RT-PCR and cloned into the prokaryotic expression vector pGEX-4T-1, then induced into E.coli with IPTG.The SDS-PAGE analysis of expressed protein demonstrated that the recombinant NS1protein of 62kD had got a high expression, which laid a good foundation for developing in molecular diagnosis and the function of the NS1gene for avian Tenbusu virus.
- avian Tenbusu virus /
- NS1gene /
- prokaryotic expression

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参考文献(20)

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