蝴蝶兰丛生芽增殖培养条件优化研究

钟海丰; 钟淮钦; 黄敏玲; 吴建设; 叶秀仙

doi:10.19303/j.issn.1008-0384.2013.07.011

蝴蝶兰丛生芽增殖培养条件优化研究

doi: 10.19303/j.issn.1008-0384.2013.07.011

钟海丰^1,2,3,
钟淮钦^1,2,3,
黄敏玲^1,2,3,
吴建设^1,2,3,
叶秀仙^1,2,3

1. 福建省农业科学院作物研究所,福建福州 350013;
2. 福建省农业科学院花卉研究中心,福建福州 350013;
3. 福建省特色花卉工程技术研究中心,福建福州 350013

基金项目:

福建省农业科学院导师制青年科技创新基金项目 (2012DQB-21)

福建省科技计划项目——省属公益类科研院所基本科研专项 (2011R1028-6)

福建省科技计划重大专项专题 (2010NZ0003-1)

详细信息

作者简介:
钟海丰 (1982-), 男, 助理研究员, 主要从事花卉品种选育与组培快繁技术研究 (zhghf008@163.com);黄敏玲 (1960-), 女, 研究员, 主要从事花卉品种选育与生物技术研究 (E-mail:pudang12@yahoo.com.cn)

中图分类号: S682.31
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- 被引次数: 0
出版历程
- 收稿日期: 2013-02-17
- 刊出日期: 2013-07-18

Optimization of Tissue Culture for Propagating Clustered Shoots of Phalaenopsis

1. Institute of Crop sciences,Fujian Academy of Agriculture Science;
2. Flowers Research Center,Fujian Academy of Agricultural Science;
3. Fujian Engineering Research Certer for Characteristic Floriculture

摘要

摘要: 以蝴蝶兰品种‘Lauluns’花梗腋芽诱导出的不定芽为试验材料, 研究芽体物理切分与摆放方式、不同无机盐浓度以及有机添加物对蝴蝶兰丛生芽增殖的影响。研究结果表明:不同的芽体物理切分与摆放方式、无机盐浓度以及有机添加物对蝴蝶兰丛生芽的增殖均有显著影响, 单芽切除生长点后横插 (PSP-3) 处理的单芽平均诱导芽数最高, 但死亡率也达到23.3%, 双芽切除生长点后横插 (PSP-6) 在控制死亡率的同时能获得较高的诱导芽数;在1/3MS¹/2MS之间的无机盐浓度比较适合于蝴蝶兰丛生芽增殖, 且种苗长势健壮;15% (v/v) 椰汁最适于蝴蝶兰丛生芽增殖, 150g·L-1的马铃薯或150g·L-1香蕉泥也可作为低成本有机添加物。
- 蝴蝶兰 /
- 丛生芽 /
- 增殖 /
- 无机盐 /
- 有机添加物 /
- 顶端优势
Abstract: Adventitious shoots induced from the peduncle auxiliary buds of Phalaenopsis‘Lauluns' were used for the study.The physical segmentation, placement mode and concentration of inorganic salt and organic additives all showed significant effects on the proliferation of Phalaenopsis with clustered shoots.Among the treatments, the greatest average number of inductive buds occurred when a bud was excised at the growth point and then grafted horizontally (PSP-3) .However, the bud's death rate was as high as 23.3%.In contrast, when the double buds were used, and followed by the horizontal grafting (PSP-6) , the budding was high, but the death rate was low.The inorganic salt concentration between 1/3MS to 1/2MS was found to be suitable for the propagating with strong seedling growth.Addition of coconut juice at 15% (v/v) level was desirable for the Phalaenopsis cluster buds proliferation, while 150g·L-1 potato juice or banana puree could be applied as low cost organic additives in the culture medium.
- Phalaenopsis /
- clustered shoots /
- proliferation /
- inorganic salt /
- organic additive /
- apical dominance

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