Biological Characteristics of the Plant Vaccin Strain FJAT-9290 Against Fusarium Wilt
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摘要: 以枯萎病植物疫苗工程菌 (尖孢镰刀菌非致病性菌株FJAT-9290) 为研究对象, 以致病性菌株FJAT-282为对照, 探索培养时间、碳氮源对2个菌株生长的影响差异, 分析非致病性尖孢镰刀菌株FJAT-9290的生物学特性。结果表明:培养过程中菌株FJAT-9290与致病性菌株FJAT-282在菌丝干重和发酵液的pH值方面无显著差异;而产孢量在第6d时达11.47×106 cfu·mL-1, 显著低于菌株FJAT-282的108.33×106 cfu·mL-1;菌株FJAT-9290的还原糖含量较菌株FJAT-282下降得快。碳源的影响表明:淀粉利于菌株FJAT-9290菌丝生长, 葡萄糖利于其产孢;木糖不利于菌株FJAT-282菌丝的生长, 果糖利于其产孢。氮源的影响表明:有机氮为氮源时菌丝生长速率快, 铵盐既不利于菌丝的生长, 也不利于产孢。氮源对菌落形态的影响比碳源大。Abstract: The biological characteristics of plant vaccin strain (nonpathogenic Fusarium oxysporum strain FJAT9290) were investigated by compare the effects of incubation time, and nutritional conditions on its growth with a pathogenic strain as a control.The results showed that there was no significant difference of the mycelium dry weight between strain FJAT-9290with the pathogenic strain FJAT-282, the sporulation of strain FJAT-9290was 1.47×106 cfu·mL-1 on the 6th day, while the strain FJAT-282is 108.33×106 cfu·mL-1, and changes of pH values had no significant effect on the culture growth.Furthermore, the concentration of reducing sugar in culture filtrate of strain FJAT-9290decreased faster than strain FJAT-282;starch was conducive to the growth of mycelium of strain FJAT-9290, glucose was conducive to its sporulation, xylose was not conducive to the growth of mycelium of strain FJAT-282, fructose was conducive to its sporulation;organic nitrogen was benefit to the growth of mycelium, ammonium salt was not conducive to the growth of hypha, but also to the sporulation.In addition, nitrogen sources exercised a greater influence on colony morphology.
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Key words:
- Fusarium oxysporum /
- growth characteristic /
- carbon source /
- carbon source
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