Effect of Temperature on the Growth and Antifungal Activity of Fusarium-wilt-disease Biocontrol Bacterium Strain FJAT-4
-
摘要: 为了解温度对枯萎病生防菌地衣芽胞杆菌菌株FJAT-4生长和抑菌作用的影响, 采用平板培养基培养、液体培养基振荡培养和含菌培养基等方法研究菌株FJAT-4在不同温度下的菌落生长特性、生长动力学及其对尖孢镰刀菌菌株FJAT-135抑制作用的浓度效应和温度效应。结果显示, 菌株FJAT-4菌落、菌体的生长都表现为在20℃以下的环境中生长缓慢、生长曲线为直线型, 在2530℃时生长较良好、生长曲线为对数型, 最佳的生长温度为3540℃;不同温度培养的菌株FJAT-4培养液及其10倍系列梯度稀释液对菌株FJAT-135都具有较强的抑制作用, 而且不同培养液或稀释液抑菌作用之间的强弱差异远小于其活菌数之间的差异;温度对菌株FJAT-4抑制菌株FJAT-135的作用有显著影响, 在15℃条件下, 菌株FJAT-4对FJAT-135的抑制作用最弱, 在2030℃条件下, 菌株FJAT-4的抑菌作用相对较强, 在35℃条件下, 菌株FJAT-4的抑菌作用最强。研究结果表明, 温度对生防菌FJAT-4的生长和抑菌作用都有显著影响, 3540℃的温度是该菌株生长和抑菌作用的最适温度。Abstract: To characterize the effect of temperature on the growth and antifungal activity of biological control strain Bacillus licheniformis FJAT-4, the growth response of strain FJAT-4at different temperature, the effect of concentration and temperature on the antifungal activity of the strain FJAT-4against Fusarium oxysporum strain FJAT-135were studied by culturing the strain on plate culture media, liquid media and culturing the strain FJAT135on the media contained strain FJAT-4.The results showed that the growth is inhibited and the growth curve is liner type under 20℃, but the growth return to normal and the growth curve is logarithmic type over 25℃, and the optimal temperature is 35-40℃.The cultured liquids of strain FJAT-4raised at different temperature and its 10 times serial dilution inhibited the growth of the strain FJAT-135effectively, and the differences among the inhibitions were far less than the differences among the concentrations of the cultured liquid.There were significant differences among effects of temperatures on the inhibitions of FJAT-4on FJAT-135, the inhibitory effect was the weakest at 15℃, and obvious at 20-30℃ and the highest at 35℃.Therefore, there were significant relationship between the temperatures and the growth and antifungal activity of strain FJAT-4, and the optimal temperature for the growth and antifungal activity was 35-40℃.
-
[1] 王政逸, 李德葆.尖孢镰刀菌致病菌营养体亲和群研究[J].浙江农业学报, 2001, 13 (1) :72-77. [2] 郝晓娟, 刘波, 谢关林.植物枯萎病生物防治研究进展[J].中国农学通报, 2005, 21 (7) :319-322, 337. [3] 肖荣凤, 刘波, 史怀, 等.生防菌哈茨木霉FJAT-9040的GFP标记及土壤定殖示踪[J].植物保护学报, 2011, 38 (6) :506-512. [4] 余超, 肖荣凤, 刘波, 等.生防菌FJAT-346-PA的内生定殖特性及对香蕉枯萎病的防治效果[J].植物保护学报, 2010, 37 (6) :493-498. [5] 郝晓娟, 刘波, 谢关林, 等.短短芽孢杆菌JK-2对番茄枯萎病的抑菌作用及其小区防效[J].中国生物防治, 2007, 23 (3) :233-236. [6] 林营志, 刘国红, 刘波, 等.香蕉枯萎病芽胞杆菌生防菌的筛选及其抑菌特性研究[J].福建农业学报, 2011, 26 (6) :1007-1015. [7] 郭景旭, 张辉, 李子钦, 等.胡麻枯萎病生防芽孢杆菌筛选及抑菌效果研究[J].中国油料作物学报, 2011, 33 (6) :598-602. [8] 车建美, 刘波, 蓝江林.短短芽孢杆菌JK-2的GFP标记及其抑菌作用[J].中国生物防治, 2010, 26 (2) :230-234. [9] 付业勤, 张科立, 潘羡心, 等.内生拮抗细菌BEB2的分子鉴定及其对香蕉枯萎病菌的抑制作用[J].热带作物学报, 2009, 30 (1) :80-85. [10] 葛慈斌, 刘波, 蓝江林, 等.生防菌JK-2对尖孢镰刀菌抑制特性的研究[J].福建农业学报, 2009, 24 (1) :29-34. [11] 唐丽娟, 纪兆林, 徐敬友, 等.地衣芽孢杆菌W10对灰葡萄孢的抑制作用及其抗菌物质[J].中国生物防治, 2005, 21 (3) :203-205. [12] BATRAKOV S G, RODIONOVA T A, ESIPOV S E, et al.A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniforrmis strain 603[J].Molecular and Cell Biology of Lipids, 2003, 1634 (3) :107-115. [13] 来航线, 杨保伟, 邱学礼, 等.9株芽孢杆菌的初步分离鉴定与拮抗性试验[J].西北农林科技大学学报, 2004, 32 (7) :93-96. [14] 李玉峰, 张笑宇, 胡俊, 等.地衣芽孢杆菌代谢物对马铃薯晚疫病菌的抑制作用[J].内蒙古农业大学学报:自然科学版, 2009, 30 (1) :10-13. [15] 马鑫, 郭宏, 张宝国, 等.地衣芽孢杆菌作为饲料添加剂的研究进展[J].中国饲料添加剂, 2011, (2) :10-13. [16] 戴求仲, 王向荣, 蒋桂韬, 等.日粮中添加芽孢杆菌制剂对黄羽肉鸡生长性能及养分利用率的影响[J].家畜生态学报, 2011, 32 (4) :18-22. [17] 马树宏.地衣芽孢杆菌胶囊加硝苯吡啶治疗肠易激综合症52例临床观察[J].中国现代药物应用, 2008, 2 (4) :46-47. [18] 郑雪芳, 葛慈斌, 林营志, 等.瓜类作物枯萎病生防菌BS2000和JK-2的分子鉴定[J].福建农业学报, 2006, 21 (2) :154-157. [19] 郝变青, 马利平, 乔雄梧, 等.拮抗菌对黄瓜枯萎病菌的室内生物活性[J].应用与环境生物学报, 2001, 7 (2) :155-157. [20] 沈萍, 陈向东.微生物学 (第2版) [M].北京:高等教育出版社, 2006. [21] 黄曦, 张荣灿, 庄军莲, 等.一株抗荔枝病原菌的枯草芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].广西科学, 2011, 18 (4) :396-401. [22] 章蕴慧, 纪兆林, 徐敬友, 等.灰葡萄孢拮抗细菌的种类鉴定及生长条件研究[J].扬州大学学报:农业与生命科学版, 2002, 23 (2) :67-70. [23] 纪明山, 王毅婧.地衣芽孢杆菌生防菌株SDYT-79发酵条件优化[J].沈阳农业大学学报, 2011, 42 (2) :164-169. [24] 胡亮亮, 徐汉虹, 廖美德.胶冻样类芽孢杆菌PS04产抗真菌物质培养条件的优化[J].华中农业大学学报, 2011, 30 (3) :276-279. [25] 张璐, 齐希猛, 刘婷婷, 等.一株拮抗芒果炭疽菌海洋细菌的鉴定和发酵培养基优化[J].广东海洋大学学报, 2011, 31 (4) :75-80. [26] 谷春涛.地衣芽孢杆菌TS-01培养条件的研究[D].北京:中国农业大学, 2004. [27] R E·布坎南, N E·吉本斯.伯杰细菌鉴定手册:第8版[M].北京:科学出版社, 1984. [28] 万利, 高翠希.生物农药使用的气象条件[J].北京农业, 2005 (8) :37. [29] 纪明山, 王建坤, 王芳, 等.温湿度对枯草芽胞杆菌B36菌株可湿性粉剂防治番茄灰霉病效果的影响[J].植物保护, 2011, 37 (1) :147-149.
点击查看大图
计量
- 文章访问数: 234
- HTML全文浏览量: 53
- PDF下载量: 3
- 被引次数: 0