Evaluation on in vitro Antioxidant Activity of Southern Wild Grape Wines
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摘要: 以不同浓度的Vc溶液为对照, 采用分光光度法分析了‘刺葡萄’和‘桂葡1号’2种南方山葡萄酒对DPPH·和·OH的清除能力以及铁氰化钾还原力。结果表明:在一定浓度范围内, 2种山葡萄酒对DPPH·和·OH的清除能力随酒样浓度的增加而逐渐增加, 二者对DPPH·的清除能力分别是2.0mg·mL-1 VC溶液的2.75倍和3.14倍, 清除·OH的能力分别是1.0mg·mL-1 VC溶液的6.98倍和7.86倍;清除速度均为:桂葡1号葡萄酒>刺葡萄酒>VC溶液。刺葡萄酒和桂葡1号葡萄酒的铁氰化钾还原力极显著高于0.5mg·mL-1的VC溶液, 但二者差异不显著。Abstract: Using Vitamin C solution as control in different concentrations, “brier grape wine”and“Gui-pu No1 wine”were studied with UV spectrophotometric assay on their scavenging activity to DPPH·, ·OH and reducing activity to potassium ferricyanide.The results showed that scavenging activity of two grape wines increased with the wine concentration.The scavenging activity to DPPH·in two wines were 2.75and 3.14time equivalent of it in Vc (2.0 mg·mL-1) , respectively, and to·OH were 6.98and 7.86time equivalent in 1.0 mg·mL-1, respectively.The scavenging speeds to free radicals were following the order in“Gui-pu No1wine”>“brier grape wine”>VC.There was no obvious difference between two wines in reducing activities to potassium ferricyanide but were higher in both wins than in Vc (0.5mg·mL-1) .
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