双孢蘑菇W192菌株液体发酵培养的研究

曾志恒; 程翊; 曾辉; 丁中文; 戴建清

doi:10.19303/j.issn.1008-0384.2013.08.008

双孢蘑菇W192菌株液体发酵培养的研究

doi: 10.19303/j.issn.1008-0384.2013.08.008

1. 福建省农业科学院食用菌研究所,福建福州,350014;
2. 福建省农业科学院,福建福州,350003

基金项目:

闽发改投资 ([2012]931)

福建省科技计划项目——省属公益类科研院所基本科研专项 (2011R1022-4)

晋安区科技计划项目 ([2012]11号)

详细信息

作者简介:
曾志恒 (1984-), 男, 研究实习员, 主要从事食用菌工厂化制种技术研究与开发;戴建清 (1972-), 男, 高级工程师, 主要从事食用菌制种工艺研究

中图分类号: S646
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出版历程
- 收稿日期: 2013-06-24
- 刊出日期: 2013-08-18

Study on Fermentation of Agaricus bisporus Strain W192

1. Edible Fungi Institute of Fujian Academy of Agricultural Sciences;
2. Fujian Academy of Agricultural Sciences

摘要

摘要: 采用响应面法对双孢蘑菇W192菌株液体发酵培养基及培养条件进行优化。通过单因子试验, 确定最佳摇瓶培养基的碳源为玉米淀粉、氮源为黄豆粉、无机盐为K2HPO4和MgSO4·7H2O, 最佳发酵条件为25℃, 摇瓶转速180r·min-1, 发酵时间7d, 起始pH6.5。在此基础上, 通过Plackett-Burman设计对影响其菌丝体生物量的8个考查因素进行评估并筛选出具有显著效应的玉米淀粉、黄豆粉和摇瓶转数为影响试验的3个重要因素。通过响应面分析法建立模型, 确定发酵培养的最适培养基和培养条件:玉米淀粉43.67g·L-1, 黄豆粉13.78g·L-1, KH2PO43.0g·L-1, MgSO4·7H2O 1.0g·L-1, 发酵温度25℃, 摇瓶转速200r·min-1, 初始pH 6.5, 发酵时间7d。在此条件下, 每100mL菌丝体生物量可达2.58g, 与初始培养条件下的菌丝体生物量相比提高了2.35倍。
- 双孢蘑菇 /
- W192 /
- 液体菌种 /
- 响应面 /
- 优化
Abstract: Culture medium and conditions for the fermentation of Agaricus bisporus strain W192 were optimized using the respond surface method.Determined by a single factor test, the optimal carbon source for the mycelial development was cornstarch;the nitrogen source, soymeal;and, the inorganic salts, K2HPO4and MgSO4·7H2O.The fermentation was best carried out in a liquid medium with an initial pH of 6.5in a flask held at 25℃and shaken constantly at 180r·min-1 for 7days.Using the Plackett-Burman design, the conditions for the A.bisporus mycelium biomass culture were evaluated.Cornstarch, soymeal and flask-shaking speed were found to be the most prominent factors that affected the fermentation.Through the respond surface analysis, a regression equation was obtained, and the fermentation conditions optimized as cornstarch at 43.67g·L-1, soymeal at 13.78g·L-1, K2HPO4at 3.0g·L-1, MgSO4·7H2O at 1.0g·L-1, incubation at 25℃, flask-shaking at 200r·min-1, medium initial pH at 6.5, and fermentation for 7days.The resultant A.bisporus mycelium biomass reached a concentration of 2.58g per 100mL, which was 2.35times higher than the orginal conditions.
- Agaricus bisporus /
- W192 /
- fermentation /
- respond surface method /
- optimization

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参考文献(10)

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