Pollen Viability and Stigma Receptivity of Large-and Abortive-seeded Longan(Dimocarpus longan)
-
摘要: 以焦核龙眼‘闽焦64-2’、‘白核’、大核龙眼‘九月乌’为材料, 采用离体萌发法, 研究了萌发时间、光照、蔗糖和硼酸浓度对花粉活力的影响, 并采用联苯胺-过氧化氢测定法研究柱头可授性。结果表明:3个龙眼品种花粉萌发率随时间延长变化不同, ‘闽焦64-2’和‘白核’的花粉活力低于‘九月乌’;光照能显著提高‘闽焦64-2’的花粉萌发率, 而对另外2个品种无显著影响;3个品种适宜的蔗糖浓度均为5%10%, 适宜的硼酸浓度不同, 分别为50100、50200和100200mg·L-1;3个品种开花当天柱头都具有可授性, 可授期均为6d。Abstract: Cultivars of the abortive-seeded longans, Minjiao 64-2and Baihe, and the large-seeded Jiuyuewu were used for the experimentation.The effects of time, light, and sucrose and boric acid concentrations on the pollen viability were determined by the seed′s in vitro germination, and the stigma receptivity, by the method of benzidine-hydrogen peroxide.The results showed that the pollen viability of the 3cultivars changed with time, and those of Minjiao 64-2and Baihe were lower than that of Jiuyuewu.Light exposure significantly increased the germination rate for Minjiao64-2, but not for Baihe or Jiuyuewu.The suitable sugar content ranged 5%-10%for all three varieties.And, the adequate boric acid content was 50-100mg·L-1 for Minjiao 64-2, 50-200mg·L-1 for Baihe, and 100-200mg·L-1 for Jiuyuewu.The stigmas of the 3cultivars appeared to be receptive from the first to the fifth day after blooming.
-
Key words:
- Longan /
- abortive-seeded /
- pollen viability /
- stigma receptivity
-
[1] 许家辉, 陈长忠, 许奇志, 等.3个焦核龙眼单株焦核遗传性试验[J].中国南方果树, 2005, 34 (3) :36-37. [2] 许家辉, 陈长忠, 蒋际谋, 等.授粉方式与疏果时期对焦核龙眼的影响[J].云南农业大学学报, 2007, 22 (1) :113-116. [3] 王翔, 刘庆华, 王奎玲, 等.耐冬山茶 (Camellia japonica L.) 花粉活力和柱头可授性研究[J].西南农业学报, 2008, 21 (4) :1078-1080. [4] 张双双, 奚晓军, 张金凤.黑杨花粉活力和柱头可授性研究[J].安徽农业科学, 2010, 38 (20) :10550-10552, 10640. [5] 王其刚, 张颢, 蹇洪英, 等.月季‘云粉’、‘云玫’的花粉活力和柱头可授性研究[J].江西农业大学学报, 2010, 32 (3) :458-461. [6] 夏瑾华, 王爱斌, 俞小凤, 等.硼酸与蔗糖对三角梅花粉生活力的影响[J].江苏农业科学, 2012, 40 (9) :186-187. [7] 许秀淡, 黄金松, 郑少泉, 等.焦核龙眼花药内游离脯氨酸及其育性[J].福建果树, 1994, (4) :9-11. [8] 赵春香, 彭乃荣, 李新福, 等.龙眼花粉生活力的研究[J].福建果树, 2002, (4) :8-10. [9] 张彩霞, 明军, 刘春.百合花粉母细胞减数分裂异常现象观测分析[J].生物学通报, 2010, 45 (5) :45-48. [10] 纪炜炜, 孟爱平, 李建强.鸭跖草科杜若 (Pollia japonica Thunb) 的减数分裂研究[J].武汉植物学研究, 2006, 24 (3) :196-200. [11] 刘丽琴, 谢江辉, 李伟才, 等.龙眼花粉母细胞减数分裂观察[J].果树学报, 2011, 28 (5) :820-824. [12] 邢艳萍, 张建, 吴杰, 等.东北地区5种蒲公英花粉离体萌发条件及特性的研究[J].沈阳农业大学学报, 2012, 43 (3) :295-299. [13] 董易, 马晓红, 沈强, 等.荷兰水仙花萌发和花粉贮藏性[J].上海交通大学学报:农业科学版, 2012, 30 (5) :25-29. [14] 王晓玉, 徐婉, 胡杏, 等.尾叶紫薇开花及花粉萌发研究[J].浙江农林大学学报, 2012, 29 (6) :966-970. [15] 康黎芳, 曹冬梅, 温喜珍, 等.外界条件对4个安祖花品种花粉萌发及生长的影响[J].山西农业科学, 2012, 40 (4) :344-347. [16] 刘星辉, 邱栋梁, 谢传龙, 等.龙眼授粉生物学研究[J].中国南方果树, 1996, 25 (1) :34-36. [17] 曾晓春, 蒋海燕, 吴晓玉, 等.茉莉酸类植物激素对水稻花粉萌发的影响[J].江西农业大学学报:自然科学版, 2003, 25 (1) :17-19. [18] 何刚, 李樊, 唐宁浩, 等.南方枣品种花粉育性与花药内源激素的变化[J].中南林业科技大学学报, 2010, 30 (10) :47-50.
点击查看大图
计量
- 文章访问数: 212
- HTML全文浏览量: 54
- PDF下载量: 2
- 被引次数: 0