液态培养红曲霉产洛伐他汀开闭环组分的研究

黄颖颖; 杨成龙; 陈源

doi:10.19303/j.issn.1008-0384.2013.08.018

液态培养红曲霉产洛伐他汀开闭环组分的研究

doi: 10.19303/j.issn.1008-0384.2013.08.018

福建省农业科学院农业工程技术研究所,福建福州,350003

基金项目:

福建省科技计划项目——省属公益类科研院所基本科研专项 (2010R1017-2)

科技部科技型中小企业技术创新基金项目 (12C26243503676)

详细信息

作者简介:
黄颖颖 (1987-), 女, 研究实习员, 硕士, 主要从事食品与发酵;杨成龙 (1964-), 男, 教授级高级工程师, 主要从事食品与发酵 (Email:yclmail@126.com)

中图分类号: TQ927
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- 文章访问数: 187
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- 被引次数: 0
出版历程
- 收稿日期: 2013-06-21
- 刊出日期: 2013-08-18

Effects of Liquid Culture for Monascus on Monacolin K and Lovastatin Production

Institute of Agricultural Engineering Technology,Fujian Academy of Agricultural Sciences

摘要

摘要: 对红曲霉产开环莫纳可林K及闭环洛伐他丁的液态发酵工艺进行研究, 探讨不同培养基成分和发酵条件对产洛伐他丁开闭环组分的影响。结果表明, 最佳的变温培养方法是先30℃培养2d后再26℃培养7d, 产生的开环莫纳可林K的比例可达87%, 比26℃恒温培养的提高了2.63倍。当培养基中添加8%木糖、3%蛋白胨、0.2%NaNO3、0.2%KH2PO4、0.35%MgSO4, 在接种量6%, 摇床转速160r·min-1, 采用变温培养方法, 得到的洛伐他汀类物质的产量最高, 且开环莫纳可林K含量比基本培养基的提高了18倍。
- 红曲霉 /
- 莫纳可林K /
- 洛伐他汀 /
- 开闭环比例 /
- 液体发酵
Abstract: The effects of medium compositions and culture conditions in Monascus liquid culture were studied on the production and the ratio of Monacolin K and Lovastatin.The optimal temperature-shift cultivation was 2days culture at 30℃followed with 7days at 26℃to increase the production of acid form Monacolin K.By this culture conditions, the proportion of acid form Monacolin K in total Monascus products reached to 87% and it was 2.63times of the ratio produced with constant temperature cultivation at 26℃.Comparing with basic culture medium, additional 8% xylose, 3% peptone, 0.2% NaNO3, 0.2% KH2PO4and 0.35% MgSO4added into the basic medium was the optimal composition that produced the highest amount of Lovastatin compounds with the culture conditions in 6%inoculums size, 160rmin-1 rotate speed at the optimal temperature-shift cultivation.The ratio of acid form Monacolin K was 18times of it produced by the basic medium.
- Monascus spp. /
- Monacolin K /
- Lovastatin /
- proportion of acid form /
- liquid culture

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参考文献(25)

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