Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector
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摘要: 以三明显性核不育为载体, 以75-1-127为Pi-9基因的供体亲本, 利用分子标记辅助方法通过连续回交把抗稻瘟病基因Pi-9导入受体亲本双抗明占中。对BC4F3代可育株进行筛选, 获得含有纯合Pi-9基因株系HP304。通过水稻DNA指纹图谱鉴定, 其遗传背景与双抗明占一致, 主要农艺性状与双抗明占没有显著差异;田间自然诱发稻瘟病抗性明显优于双抗明占。实践证明, 利用三明显性核不育水稻转导抗稻瘟病Pi-9基因具有可行性。Abstract: Transduction of rice blast resistance gene Pi-9 for rice was studied with Sanming dominant genic male sterile rice as the vector and the rice blast resistance 75-1-127 with Pi-9 gene as the donor.Pi-9 gene was introduced into the rice restore Shuangkangmingzhan by successive backcrosses with supporting molecular markers.The results indicated that the pure lines of HP304 with Pi-9 gene similar to Shuangkangmingzhan had been selected from the fertile plants of BC4F3.The basic genetic background of HP304 had been proved to be the same to that of Shuangkangmingzhan. The main agronomic traits of HP304 had no significant difference with Shuangkangmingzhan.And the resistance identification in field showed that the resistant level of HP304 was significantly higher than that of Shuangkangmingzhan.The above demonstrated that it was available to transfer Pi-9 gene into rice using Sanming dominant geneic male sterile as the vector.
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Key words:
- rice /
- sanming dominant genic male sterile /
- blast /
- Pi-9
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