A Study of Antigenic Relativity on Muscovy Duck Parvovirus and Goose Parvovirus
-
摘要: 应用微量交叉中和试验 (NT) 和胶乳凝集抑制试验 (LPAI) 比较番鸭细小病毒、鹅细小病毒和番鸭源鹅细小病毒之间的抗原相关性。结果显示:3种病毒与同源抗血清的中和效价和LPAI效价均明显高于异源血清;番鸭细小病毒与番鸭源鹅细小病毒、鹅细小病毒之间的R值均小于0.1;番鸭源鹅细小病毒与鹅细小病毒的R值大于0.25。结果表明番鸭细小病毒与鹅细小病毒 (番鸭源或鹅源) 的抗原相关性较低, 提示番鸭源鹅细小病毒与鹅细小病毒属于同一血清型的不同亚型。Abstract: Antigenic relationship of Muscovy duck parvovirus (MPV F strain) , Muscovy duck origin goose parvovirus (MDGPV PT strain) and Goose parvovirus (GPV GD strain) were determined by virus crossneutralization tests (NT) and latex agglutination inhibition test (LPAI) .The results showed that neutralization titer and LPAI titers of homologous antiserum were significantly higher than heterologous serum;R values of MPV compared with MDGPV and GPV were less than 0.1.R value between MDGPV and GPV was greater than 0.25.The results showed that MPV has low antigenic correlation with GPV and MDGPV, which indicated that antigenic variations are obviously existed between MPV and the other two, and showed that GPV and MDGPV belong to different subtypes of the same serotype.
-
[1] 程由铨, 林天龙, 胡奇林, 等.雏番鸭细小病毒病病毒分离和鉴定[J].病毒学报, 1993, 9 (3) :228-235. [2] 胡奇林, 吴振允, 周文谟, 等.雏番鸭细小病毒病的流行病学调查[J].中国兽医杂志, 1993, 19 (6) ;7-8. [3] 方定一.小鹅瘟介绍[J].中国畜牧兽医, 1962, (8) :19-20. [4] 程晓霞, 陈少莺, 朱小丽, 等.番鸭小鹅瘟病毒的分离与鉴定[J].福建农业学报, 2008, 23 (4) :355-358. [5] 殷震, 刘景华.动物病毒学[M].第2版.北京:科学出版社, l997:l165-l168. [6] 林天龙, 程由铨, 胡奇林, 等.雏番鸭细小病毒病的研究lV.胶乳凝集试验在雏番鸭细小病毒病诊断中的应用[C]//中国畜禽传染病——中国免疫学会兽医免疫委员会学术讨论会论文集, 1992:71-74. [7] 秦卓明, 徐怀英, 欧阳文军, 等.新城疫不同毒株交叉鸡胚中和指数及其与F和HN基因变异的相关性[J].微生物学报, 2008, 48 (2) :226-233. [8] LE GALL-RECULE G, JESTIN V.Biochemical and genomic characterization of muscovy duck parvovirus[J].Arch Virol, 1994, 139 (1-2) :121-131. [9] ZADORI Z, ERDEI J, NAGY J.Characteristics of the genome of goose parvovirus[J].Avian Pathol, 1994, 23:359-364. [10] 程由铨, 胡奇林, 陈少莺, 等.番鸭细小病毒和鹅细小病毒生化及基因组特性的比较[J].中国兽医学报, 2001, 21 (5) :429-433. [11] 王劭, 朱小丽, 林锋强, 等.番鸭源小鹅瘟病毒PT株VP基因的克隆与序列分析[J].农业生物技术学报, 2012, 20 (1) :79-84.
点击查看大图
计量
- 文章访问数: 230
- HTML全文浏览量: 52
- PDF下载量: 4
- 被引次数: 0