Genetic Analysis for Minor Effect Restoring Gene for CMS-WA in Rice(Oryza sativa L.)
-
摘要: 为分析水稻育性微效恢复基因的表型遗传, 以套袋自交结实率、嵌合颖花率和黑染花粉率为指标考查野败型 (WA) 不育系珍汕97A、龙特浦A和京福1A的育性在3个转育高世代中的表现。研究结果:黑染花粉粒的出现与微效恢复基因有关, 导致不育系自交结实, 从而产生杂株;采取遗传提纯等方法可以排除部分微效恢复基因, 达到提纯和去杂的目的, 若要完全将其排除, 在理论和实践上是不可能的;嵌合颖花率适合作为微效恢复基因遗传分析的量化指标, 为进一步研究其分子遗传提供基础。Abstract: For the genetic analysis of minor effect restoring gene in rice, the CMS lines of Zhenshan 97A, Longtepu A and Jinfu 1A with different fertile levels were used as materials to investigate three sterility indexes, bagged seed setting rate, dark pollen rate by I2-IK dyed and rate of florets with fertile anthers.The results showed that the appearance of abnormal plants in CMS-WA was due to partial restoration of self-fertility few floras caused by minor effect restoring gene.Adopted the method of genetic improvement could eliminate part of minor effect restoring gene to get purification and elimination, but not completely ruled out.This index of rate of florets with fertile anthers could be use as a suitable tool to study heredity laws of minor effect restoring genes and its further molecular mechanism.
-
Key words:
- rice /
- wild abortive /
- cytoplasmic male sterile line /
- restoring gene with minor effect
-
[1] 曾千春, 周开达, 朱祯, 等.中国水稻杂种优势利用现状[J].中国水稻科学, 2000, 14 (4) :243-246. [2] 朱华高.杂交稻AB类杂株产生原因及不育系育性变化原因的初步研究[J].作物学报, 1993, 9 (2) :139-142. [3] 郑秀萍, 张功宙.龙特甫A杂株来源及其分析[J].杂交水稻, 1998, 13 (1) :8-11. [4] 周天理, 沈锦骅, 叶复初.野败型杂交籼稻的育性基因分析[J].作物学报, 1983, 9 (4) :6-13. [5] 傅强.野败型杂交籼稻恢复基因的遗传研究[J].福建农学院学报, 1985, (3) :194-202. [6] 高明尉.野败型杂交籼稻基因型的初步分析[J].遗传学报, 1981, 8 (1) :66-74. [7] 黎垣庆.IR24恢复基因遗传的系谱分析[J].中国农业科学, 1985, (1) :24-31. [8] 周天理, 郑秀萍, 陈金泉, 等.水稻不育系中杂株的来源及遗传分析[J].作物学报, 1992, 18 (1) :9-16. [9] 雷捷成, 游年顺, 郑秀平.野败型水稻雄性不育系保持系选育的遗传分析[J].中国农业科学, 1984, (5) :30-34. [10] 雷捷成, 游年顺, 郑秀平.野败水稻雄性不育系保持系选育的实践和理论[J].福建省农科院学报, 1986, 1 (2) :16-21. [11] 雷捷成, 游年顺, 黄利兴, 等.籼型三系不育系选育的实践和理论[J].福建农业学报, 2000, 15 (S1) :136-140. [12] 游年顺, 黄利兴, 雷上平, 等.水稻微效恢复基因与不育系选育研究[J].江西农业大学学报, 2003, 25 (4) :487-492. [13] 富昊伟, 李友发, 马兴华, 等.“三系”不育系微效恢复基因表现型研究[J].作物研究, 2010, 24 (1) :8-11.
点击查看大图
计量
- 文章访问数: 205
- HTML全文浏览量: 35
- PDF下载量: 3
- 被引次数: 0