Comparison of ISSR and SRAP Markers on the Genetic Diversity Analysis of Dioscoreae
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摘要: 以90份山药种质资源为材料, 从多态性检测水平、遗传多样性检测水平、遗传距离分析和聚类分析4个方面对ISSR与SRAP揭示山药种质资源遗传多样性的差异进行分析。结果表明:SRAP标记的扩增谱带总数、平均扩增谱带数、平均多态性谱带数都远高于ISSR标记, 遗传距离范围也比ISSR标记更广, 但多态性比率差异不大;2种标记在检测遗传多样性和种群间的分化程度方面较为相似, 二者结果均表明山药种质资源总的遗传变异中约有52%变异存在于种群间, 约48%的变异存在于种群内;从聚类分析的结果上看, ISSR标记在以遗传距离为0.13时将山药资源分为4个类群, 而SRAP标记以遗传距离为0.18时将其分为5个类群。综合4个方面比较结果及SRAP标记和ISSR标记自身的特点, 认为SRAP标记更适合用于山药遗传多样性分析。Abstract: To analysis the genetic diversity of 90 germplasm, ISSR and SRAP markers were studied by polymorphisms, genetic diversity, genetic distance and cluster analysis.Our results showed that the total number of amplified bands, the average number of amplified bands and the average number of polymorphic bands obtained from SRAP marker are more than those marked by ISSR, and the range of genetic distance marked by SRAP was broader.However, there had no difference of the polymorphism ratios of the two markers.Furthermore, there had 52% variations between the populations and nearly 48% variations in populations, suggesting that the two markers are similar in genetic diversity analysis.Cluster analysis marked by ISSR showed that the dioscorea resources are classified into 4groups with a genetic distance of 0.13, and the results marked from SRAP showed that they are classified into 5 groups with a genetic distance of 0.18.In conclusion, our results suggest that SRAP marker is more suitable for the analysis on the genetic diversity of dioscoreae.
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Key words:
- Dioscorea polystachyaTurcz. /
- ISSR /
- SRAP /
- genetic diversity
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[1] 中国植物志编委会.中国植物志 (第十六卷第一分册) [M].北京:科学出版社, 1985:60-105. [2] 聂凌鸿, 宁正祥.山药的开发利用[J].中国野生植物源, 2002, 21 (5) :17-19. [3] 丁嬴.山药穿山龙[M].北京:中国中医药出版社, 2001:28-29. [4] CHUN O K, KIM D O, SMITH N, et al.Daily consumption of phenolics and total antioxidant capacity from fruit and vegetables in the American diet[J].Journal of Science Food Agriculture, 2005, 85:1715-1724. [5] RUBATZKY, YAMAGUCBI.World vegetables:principles, production, and nutritive values (second edition) -New york[J].International Thomson Publishing, 1997:162-182. [6] 巩庆平, 程广, 袁良敏.山药标准化栽培技术[M].北京:中国农业出版社, 2004. [7] DWECK A C.The Wild Yam-review of Dioscorea species[J].Personal Care Magazine, 2002, 3 (3) :7-9. [8] 周生茂.山药不同基因型地下块茎糖类和酚类物质形成、调控及相关基因分离的研究[D].杭州:浙江大学, 2009. [9] 廖俊奎.台湾产薯蓣属 (薯蓣) 科之分类研究[D].台湾:中山大学, 2000. [10] 沈程文, 宁正祥, 黄建安, 等.基于表型参数及SRAP标记的广东茶种质遗传多样性[J].应用生态学报, 2009, 20 (7) :1551-1558. [11] FERRIOL M, PIOB, NUEZ F.Morphological and molecular diversity of a collection of Cucurbita max in a landraces[J].Journal of the American Society for Horticultural Science, 2004, 129 (1) :60-69. [12] 罗光佐, 施季森, 尹佟明, 等.利用RAPD标记分析北美鹅掌楸与鹅掌楸种间遗传多样性[J].植物资源与环境学报, 2000, 9 (2) :9-13. [13] LI G, QUIROS C F.Sequence related amplified polymorphism (SRAP) , a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics, 2001, 103:455-461. [14] WANG G, PAN J-S, LI X-Z, et al.The construction of genetic link-age map and collateral gene mapping of cucumber by SRAP markers[J].Science in China Series C, 2004, 34 (6) :510-516. [15] 周延清, 景建洲, 李振勇, 等.用ISSR标记技术分析山药品种遗传多样性[J].实验生物学报, 2005, 38 (4) :324-330. [16] 华树妹, 涂前程, 雷伏贵.福建山药种质资源遗传多样性的RAPD分析[J].植物遗传资源学报, 2009, 10 (2) :195-200. [17] 蔡金辉, 严渐子, 黄晓辉, 等.山药品种资源的分类研究[J].江西农业大学学报, 1999, 21 (1) :53-57. [18] 周延清, 景建洲, 李振勇, 等.用ISSR标记技术分析山药品种遗传多样性[J].实验生物学报, 2005, 38 (4) :324-330. [19] 黄玉仙, 黄姗, 梁康迳, 等.基于SRAP标记的山药种质资源遗传多样性分析[J].中国野生植物资源, 2011, 30 (6) :48-54. [20] 郑志雷.厚朴遗传多样性研究及指纹图谱的构建[D].福州:福建农林大学, 2010. [21] 许先松, 刘志钦, 林晓丹, 等.基于形态及SRAP标记的辣椒资源遗传多样性及亲缘关系比较[J].福建农林大学学报, 2011, 40 (1) :48-53. [22] 沈程文, 黄建安, 赵世浩, 等.用SRAP和ISSR标记分析广东省冬虫草夏草遗传多样性[J].核农学报, 2010, 24 (5) :948-955. [23] 彭方仁, 吴莺莺, 郝明灼, 等.利用ISSR和SRAP标记分析油茶遗传多样性[J].南京林业大学学报:自然科学版, 2012, 36 (5) :19-25.
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