福建正红菇遗传多样性分析

肖冬来; 陈宇航; 杨菁; 黄小菁

doi:10.19303/j.issn.1008-0384.2013.09.014

福建正红菇遗传多样性分析

doi: 10.19303/j.issn.1008-0384.2013.09.014

福建省农业科学院食用菌研究所,福建福州,350003

基金项目:

福建省自然科学基金项目 (2011J05056)

福建省农业科学院青年科技人才创新基金项目 (2010BS-1)

详细信息

作者简介:
肖冬来 (1981-), 男, 助理研究员, 主要从事菌根菌和分子生物学研究 (Email:xdljiangsu@163.com)

中图分类号: S646.9
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出版历程
- 收稿日期: 2013-06-13
- 刊出日期: 2013-09-18

Phylogenetic Diversity of Russula griseocarnosa from Fujian

Edible Fungi Institute of Fujian Academy of Agricultural Sciences

摘要

摘要: 以采自福建省山区的正红菇Russula griseocarnosa为研究材料, 进行ITS (internal transcribed spacer) 、RPB2 (the second largest subunit of the nuclear RNA polymerase enzyme II) 保守区段克隆测序和序列同源性比对。将从GenBank中获得的部分红菇属物种ITS和RPB2序列与正红菇序列通过最大简约法进行系统发育分析。基于ITS序列构建的系统发育树表明, 福建正红菇与云南大红菌Russula griseocarnosa X.H.Wang, Zhu L.Yang&Knudsen, sp.nov.间序列差异较小, 亲缘关系较近, 以99%的支持率聚为一簇。福建正红菇与欧洲红菇Russula vinosa及玫瑰红菇Russula rosea间序列差异较大, 亲缘关系较远, 在系统发育树上聚为不同分枝。基于RPB2序列构建的系统发育树也表明福建正红菇与云南大红菌聚为一簇 (支持率94%) , 而与玫瑰红菇聚为不同分支。
- 正红菇 /
- 菌根菌 /
- 遗传多样性 /
- ITS /
- RPB2
Abstract: Fruiting bodies of Russula griseocarnosa were collected in Fujian to study the mushroom′s phylogenetic diversity.Two gene regions of ITS (internal transcribed spacer) and RPB2 (the second largest subunit of the nuclear RNA polymerase enzyme II) were cloned.The phylogenetic diversity was examined according to the maximum parsimony trees with sequences of several other Russula species.It showed that, based on the ITS sequences, R.griseocarnosafound in the province clustered with R.griseocarnosa X.H.Wang, Zhu L.Yang & Knudsen, sp.nov.found in Yunnan, with a bootstrap value of 99%.The differences among R.griseocarnosa, R.vinosa and R.rosea were apparent, with a bootstrap value below 50%.The maximum parsimony tree based on the RPB2 sequences also showed that R.griseocarnosa clustered with R.griseocarnosa X.H.Wang, Zhu L.Yang & Knudsen, with a bootstrap value of 94%.The distinction was also apparent, with a bootstrap value less than 50%.
- Russula griseocarnosa /
- mycorrhizal fungi /
- phylogenetic diversity /
- ITS /
- RPB2

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参考文献(9)

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