光照对兴国灰鹅PRL基因表达的影响

武艳平; 霍俊宏; 熊新华; 谢明贵; 康昭风; 周泉勇; 陈受金; 曾文; 韦启鹏; 谢金防

doi:10.19303/j.issn.1008-0384.2013.10.002

光照对兴国灰鹅PRL基因表达的影响

doi: 10.19303/j.issn.1008-0384.2013.10.002

1. 江西省农业科学院畜牧兽医研究所,江西南昌,330200;
2. 中牧实业股份有限公司江西生物药厂,江西南昌,330200;
3. 江西省兴国县兴国灰鹅生产办公室,江西兴国,342400

基金项目:

国家水禽产业技术体系专项 (CARS-43-33)

江西省科技支撑计划项目 (2010jx00126)

详细信息

作者简介:
武艳平 (1976-), 女, 博士, 副研究员, 主要从事家禽遗传育种研究 (E-mail:wyp_0902@126.com);谢金防 (1962-), 男, 研究员, 主要从事家禽育种研究 (E-mail:xiejf166@163.com)

中图分类号: S835.3
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- 被引次数: 0
出版历程
- 收稿日期: 2013-05-28
- 刊出日期: 2013-10-18

Effect of Light Exposure on PRL Gene Expression of Xingguo Gray Goose

1. Insititute of Animal Husbandry and Veterinary Medicine,Academy of Agricultural Sciences;
2. Jiangxi Biologic Factory,China Animal Husbandry Industry Company Limited;
3. Xingguo Gray Goose Production Office

摘要

摘要: 为调节兴国灰鹅繁殖的季节性, 选择健康且体重均匀的兴国灰鹅进行短光照处理, 分析光照处理对兴国灰鹅的垂体、下丘脑、卵巢和输卵管4个组织催乳素 (PRL) 基因表达的影响。研究结果表明, PRL基因mRNA在垂体、下丘脑、输卵管和卵巢的表达依次降低, 垂体的表达最高, 卵巢最低。与自然光照处理 (对照) 相比, 试验组垂体和输卵管PRL的表达显著低于对照组 (P<0.05) , 下丘脑和卵巢PRL的表达在试验组和对照组间无明显差异, 表明光照处理的兴国灰鹅在产蛋期PRL基因表达明显减少。
- 光照 /
- 兴国灰鹅 /
- PRL /
- 基因表达
Abstract: Randomly selected healthy Xingguo grey geese with a male to female ratio of 1to 5were divided into two groups.The treatment group was exposed to artificial light, and the control under natural conditions.The pituitary, hypothalamus, ovary and oviduct were collected from the geese for tests.The mRNA expression of the PRL genes was found to decrease in all four organs, with the highest expression in the pituitary and the lowest in the ovary of the geese.The treatment group had a significantly lower expression in the pituitary and oviduct than control (P<0.05) , but did not differ significantly in the hypothalamus and ovary.
- light exposure /
- Xingguo gray goose /
- PRL /
- gene expression

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参考文献(17)

[1]	施振旦, 孙爱英.鹅反季节调控技术[J].农村养殖技术, 2011, (21) :9-10.
[2]	孙爱东, 梁少东, 施振旦.广东省鹅反季节繁殖和生产的经济效益分析[J].中国家禽, 2005, 27 (4) :17-19.
[3]	SHARP P J, BLACHE D.A neuroendocrine model for prolactin as the key mediator of seasonal breeding in birds under long-and short-day photoperiods[J].Cannadian Journal of Physiology and Pharmacology, 2003, 81:350-358.
[4]	李朝国, 赵振洪, 王定国, 等.南方高山地区种鹅反季节生产的措施与方法[J].中国家禽, 2005, 27 (20) :16-17.
[5]	施振旦, 黄运茂, 孙爱东, 等.光照对广东灰鹅繁殖季节性影响的研究[J].广东农业科学, 2005, (3) :72-75.
[6]	黄运茂.马岗鹅繁殖活动的调控研究[D].广州:华南农业大学, 2005.
[7]	苏伟岳, 杨承忠, 唐受辛.光照对马岗鹅繁殖特性的调控作用[J].中国畜牧杂志, 2006, 42 (15) :24-25.
[8]	唐珊珊, 朱基美.控制光照对非繁殖期种公鹅睾丸形态和组织学结构变化的研究[J].华南农业大学学报, 1989, 10 (1) :59-64.
[9]	黄运茂, 施振旦, 田允波.催乳素促进禽类卵泡发育的研究进展[J].仲恺农业技术学院学报, 2008, 21 (4) :66-70.
[10]	LIVAK K J, SCHMITTGELL T D.Analysis of relative gene expression data using real time quantitative PCR and the 2-ΔΔCt method[J].Methods, 2001, 25:402-408.
[11]	LIW L, LIU Y, YU Y C, et al.Prolactin plays a stimulatory role in ovarian follicular development and egg laying in chicken hens[J].Domestic animal endocrinology, 2011, 41 (2) :57-66.
[12]	LI H F, SHU J T, DU Y F, et al.Analysis of the genetic effects of prolactin gene polymorphisms on chicken egg production[J].Molecular Biology Reports, 2013, 40 (1) :289-294.
[13]	XU H P, ZENG H, ZHANG D X, et al.Polymorphisms associated with egg number at 300days of age in chickens[J].Genetics and molecular research:GMR, 2011, 10 (4) :2279-2289.
[14]	LIU Z, SHI Z D, LIU Y, et al.Molecular cloning and characterisation of the Magang goose prolactin gene[J].General and comparative endocrinology, 2008, 155 (1) :208-216.
[15]	KANG B, GUO J R, YANG H M, et al.Differential expression profiling of ovarian genes in prelaying and laying geese[J].Poultry Science, 2009, 88 (9) :1975-1983.
[16]	SHARP P J.Strategies in avian breeding cycles[J].Animal Reproduction Science, 1996, 42:505-513.
[17]	SHARP P J, BLACHE D.A neuroendocrine model for prolactin as the key mediator of seasonal breeding in birds under long-and short-day photoperiods[J].Canadian journal of physiology and pharmacology, 2003, 81 (4) :350-358.