Induction,Subculture and Maintenance of Fuzhou Jasmine,Jasminum sambac (Linn.) Aiton
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摘要: 以福州茉莉花茎段或花器官组织为外植体, 进行愈伤组织诱导、继代增殖和长期保持等方面的研究。结果表明, 在光照条件为1 5002 000lx下, 能够诱导获得6种类型的愈伤组织, 愈伤组织诱导适宜培养基为MS+BA 2.0mg·L-1+NAA 0.2 mg·L-1+蔗糖30g·L-1, 诱导率可达100%;同时在MS+BA 2.0mg·L-1+NAA 0.2mg·L-1+蔗糖30g·L-1培养基中, 能长期继代保持茉莉花愈伤组织, 并维持旺盛的生长能力。Abstract: Plantlets of Fuzhou jasmine, Jasminum sambac (Linn.) Aiton, were obtained by using the stems and floral organ of the plant as explants.The callus induction, subculture and long-term maintenance of the plantlets were investigated on media with varied concentrations of phytohormone.The results indicated that the optimal light density for the culture was 1 500-2 000lx, and that 6different types of callus could be formed by varying the phytohormone concentration.The medium consisting of MS, 2.0mg BA/L, 0.2mg NAA·L-1 and 30g sucrose ·L-1 yielded a 100%induction rate, and at the same time, was the best for the callus subculture multiplication, as well as the growth of the induced plants.
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Key words:
- jasmine[Jasminum sambac (Linn.) Aiton] /
- explant /
- callus /
- induction /
- subculture
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