Abstract: The regular growth of flue-cured tobacco is affected by phosphorus supply.The pot experiment was conducted to study effects of phosphorus rate in tobacco-planting soil with different available phosphorus levels on the growth and dry matter accumulation of flue-cured tobacco.The results showed that, in moist sandy land (available phosphorus 13.29 mg·kg-1, soil phosphorus adsorption maximum 67.3 mg·kg-1) , phosphorus application was 1.35g·pot-1 (P4) which the agronomic traits of flue-cured tobacco exhibited higher levels, the dry matter accumulation of flue-cured tobacco was not significant with P5treatment and could meet the demand of the regular growth of the flue-cured tobacco.In yellow clayey soil (available phosphorus 28.66 mg·kg-1, phosphorus adsorption maximum 168.2mg·kg-1) , the agronomic traits of flue-cured tobacco which applied 2.00 g·pot-1 (P5) phosphorus was at the best level significantly higher than other treatments.The dry matter accumulation of flue-cured tobacco could reach the maximum value 79.03g·pot-1 significantly higher than P4 treatment, which could meet the demand of the regular growth of the flue-cured tobacco.In paddy field (available phosphorus 54.50mg·kg-1, phosphorus adsorption maximum 104.0mg·kg-1) , the agronomic traits of fluecured tobacco which applied 1.35g·pot-1 (P4) phosphorus was at best level significantly higher than others.The dry matter accumulation of flue-cured tobacco could reach the maximum value 95.04g·pot-1, which could meet the demand of the regular growth of the flue-cured tobacco, with the same phosphorus applied, the agronomic traits and dry matter accumulation of flue-cured tobacco were paddy field>yellow clayey soil>moist sandy land. Which showed that phosphorus fertilizer rate in tobacco-planting soil with different available phosphorus levels was significantly related to the growth of flue-cured tobacco.