Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves
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摘要: 研究超声法提取木豆叶总黄酮的提取工艺, 通过单因素、L9 (34) 正交实验, 探讨超声时间、乙醇浓度、料液比对木豆叶总黄酮得率的影响。结果表明, 木豆叶总黄酮超声提取最佳条件为:超声时间20min, 乙醇浓度70%, 料液比为1∶30, 总黄酮得率达到3.65%。该检测方法稳定性高, 重现性好, 加样回收率为99.01%~102.72%。Abstract: Extracting total flavonoids in pigeon pea leaves by ultrasonic method.Analysis the factors which ultrasonic extraction time, the concentration of ethanol and the solid-liquid ratio condition and L9 (34) orthogonal, to find the optimal conditions of extraction of total flovonoids in pigeon pea leaves.From the orthogonal results of ultrasonic extraction, the optimal conditions of extraction of total flavonoids in pigeon pea leaves are as following: 20min ultrasonic extraction 70%ethanol, 1∶30solid-liquid ratio.With above extraction condition the yield of total flavonoids is 3.65%.The average recovery of the detection method is 99.01%-102.72%, the method has excellent stability and repeatability.
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Key words:
- pigeon pea leaves /
- total flavonoids /
- ultrasonic extraction /
- extraction process
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