葡萄柚果汁延缓果蝇衰老作用的研究

王琦; 郑亚凤; 高慧颖; 余亚白

doi:10.19303/j.issn.1008-0384.2013.12.015

葡萄柚果汁延缓果蝇衰老作用的研究

doi: 10.19303/j.issn.1008-0384.2013.12.015

1. 福建省农科院农业工程技术研究所,福建福州,350003;
2. 福建农林大学食品科学院,福建福州,350002

基金项目:

农业部948重大项目子课题 (2012-Z30)

详细信息

作者简介:
王琦 (1981-), 男, 硕士, 研究方向:食品生物技术 (E-mail:nkywq@163.com);余亚白 (1952-), 女, 研究员, 主要从事台湾果树引进与创新利用研究

中图分类号: S666.3
计量
- 文章访问数: 184
- HTML全文浏览量: 59
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2013-10-12
- 刊出日期: 2013-12-18

Effects of Grapefruit Juice on anti-aging for Drosophila melanogaster

1. Institute of Agricultural Engineering,Fujian Academy of Agricultural Sciences;
2. College of Food Science,Fujian Agriculture and Forestry University

摘要

摘要: 通过果蝇生存试验研究葡萄柚果汁的体内抗氧化功效和延缓衰老作用。在果蝇培养基中分别加入5%、10%、20%的葡萄柚果汁, 测定果蝇平均寿命和最高寿命;并测定果蝇组织匀浆中的超氧化物歧化酶 (SOD) 值和丙二醛 (MDA) 含量。结果显示葡萄柚果汁低、中、高剂量组对雄果蝇平均寿命的延长率分别为8.24%、20.06%和13.50%, 对雌果蝇平均寿命的延长经分别为12.04%、24.93%和19.33%, 差异均达极显著水平。不同浓度的葡萄柚果汁均能提高雄、雌果蝇体内的SOD活性, 降低MDA含量水平。其中添加10%果汁效果最好, 与果蝇生存试验结果一致。
- 葡萄柚 /
- 果蝇 /
- 延缓衰老 /
- 超氧化物歧化酶 /
- 丙二醛
Abstract: Using fruit fly (Drosophila melanogaster) survival experiment, we investigated the antioxidant and antiaging functions of grapefruit juice in vitro.Low dose (5%) 、middle dose (10%) and high dose (20%) of grapefruit juice were added into the basic medium for fruit fly respectively, the average lifespan and the highest lifespan of the fruit flies fed in three groups were calculated.Moreover, SOD activity and MDA content in the homogenate of fruit fly body were determined.The results showed that, with low, middle and high dosages, the grapefruit juice significantly prolonged the average lifespan of male fruit flies by 8.24%, 20.06%and 13.50%, respectively, while 12.04%, 24.93% and 19.33% for it of the female fruit flies, respectively.Feeding grapefruit juice in certain concentrate could improve SOD activity and reduce MDA content for both male and female fruit flies.The data from middle dose group showed the best effects which coincide with the results of fruit fly survival experiment.
- grapefruit /
- Drosophila melanogaster /
- anti-aging /
- SOD /
- MDA

HTML全文

参考文献(17)

[1]	GUTTERIDGE J M C, HALLIWELL B.Free radicals and antioxidants in the year 2000:A historical look to the future[J].Ann N Y Acad Sci, 2000, 899:136-147.
[2]	张克烽, 张子平, 陈芸, 等.动物抗氧化系统中主要抗氧化酶基因的研究进展[J].动物学杂志, 2007, 42 (2) :153-160.
[3]	IGUAL M, GARCIA-MARTINEZ E, CAMACHO M M, et al.Changes in Flavconoids content of grapefruit juice by thermal treatment and storage[J].Innovative Food Science and Emerging Technologies, 2011, 12:153-162.
[4]	KLIMCZAK I, MALECKA M, SZLACHTA M, et al.Effect of storage on the content of polyphenols, vitaminC and the antioxidant activity of orangejuices[J].Journal of Food Composition and Analysis, 2007, 20:313-322.
[5]	WANG Y C, CHUANG Y C, HAU H W.Theavonoid, carotenoid and pectin content in peels of citrus cultivated inTaiwan[J].Food Chemistry, 2008, 106:277-284.
[6]	ROSSA S A, ZISKA D S, ZHAO K, et al.Variance of common flavonoids by brand of grapefruit juice[J].Fitoterapia, 2008, 71:154-161.
[7]	PTTERSON J J, BEECHER G R, BHAGWAT S A, et al.Flavanones in grapefruit, lemons, and limes:Acompilation and review of the data from the analytical literature[J].Journal of Food Composition and Analysis, 2006, 19:S74-S80.
[8]	王琦, 余亚白, 赖呈纯, 等.几种台湾水果的抗氧化能力研究[J].福建农业学报, 2010, 25 (6) :703-706.
[9]	HELFAND S L, ROGINA B.Molecular genetics of aging in the fly:is this the end of the beginning[J].Bioessays, 2003, 25:134-141.
[10]	史建勋, 茅海琼, 胡卓逸.麦冬多糖对家蚕、果蝇寿命和对衰老小鼠单胺氧化酶及血清溶血素的影响[J].中国药杂志, 2009, 34 (13) :1737-1740.
[11]	周波, 王晓红, 郭连营, 等.玉米紫色植株花色普色素延缓衰老的功能[J].中国临床康复, 2006, 10 (19) :138-140.
[12]	王玉华, 叶加, 李长龄, 等.冬虫夏草提取物抗衰老实验研究[J].中国中药杂志, 2004, (29) :773-776.
[13]	王菊凤, 李鹄鸣.蛹虫草多糖对果蝇寿命影响的研究[J].中国野生植物资源, 2008, 27 (3) :38-42.
[14]	葛斌, 许爱霞, 杨杜华.黄芪多糖抗衰老作用机制的研究[J].中国医院药学杂志, 2004, 24 (10) :610-612.
[15]	郑亚凤, 王琦, 黄志伟, 郑金贵.灰树花多糖延缓果蝇衰老作用[J].福建农林大学学报:自然科学版, 2010, 35 (6) :625-627.
[16]	林斌, 余亚白, 赖呈纯, 等.晚熟耐贮葡萄柚品种——“闽农1号”的选育[J].福建果树, 2006, (2) :9-10.
[17]	王琦, 余亚白, 陈源, 等.闽农1号葡萄柚的品种特性及其推广前景[J].福建农业科技, 2008, (5) :29-30.