甘薯羽状斑驳病毒（SPFMV）ELISA鉴定及RT-PCR检测方法的建立

许泳清; 李华伟; 刘中华; 邱永祥; 罗文彬; 纪荣昌; 汤浩; 邱思鑫; 余华

doi:10.19303/j.issn.1008-0384.2013.12.016

甘薯羽状斑驳病毒（SPFMV）ELISA鉴定及RT-PCR检测方法的建立

doi: 10.19303/j.issn.1008-0384.2013.12.016

1. 福建省农业科学院作物研究所,福建福州,350013;
2. 福建省农业科学院中心实验室,福建福州,350003

基金项目:

国家甘薯产业技术体系项目 (CARS-11-B-10-2013)

福建省财政专项——福建省农业科学院创新团队建设项目 (CXTD-1-1301)

详细信息

作者简介:
许泳清 (1980-), 女, 助理研究员, 主要从事甘薯组织培养与病毒检测研究 (E-mail:qingqing0722@126.com);邱思鑫 (1974-), 男, 博士, 研究员, 主要从甘薯病虫害防控技术研究 (E-mail:qiusixin@yahoo.com.cn);余华 (1957-), 女, 研究员, 主要从事甘薯病虫害防控技术研究 (E-mail:yuhua@faas.cn)

中图分类号: S435.31
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出版历程
- 收稿日期: 2013-10-21
- 刊出日期: 2013-12-18

ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus

1. Institute of Crop Sciences,Fujian Academy of Agricultural Sciences;
2. Central Laboratory,Fujian Academy of Agricultural Sciences

摘要

摘要: 利用双抗体酶联免疫吸附测定法 (DAS-ELISA) 对采自福建省51份甘薯叶片进行检测, 结果显示51份样品中有24份SPFMV呈阳性, 阳性率为47.06%, 其中泉州样品SPFMV阳性率最高, 为71.40%, 其次为南安和龙岩的样品, 阳性率均为50%。根据GenBank中公布的SPFMV外壳蛋白 (CP) 基因序列保守区域设计了1对特异性引物, 通过RT-PCR反应程序的优化建立了能检测SPFMV的检测方法。该检测方法能够扩增出SPFMV特异性片段, 片段大小为441bp, 测序结果表明, SPFMV序列与参考序列的同源性92%⁹7%。
- 甘薯 /
- 病毒 /
- 甘薯羽状斑驳病毒 /
- ELISA /
- RT-PCR
Abstract: 51samples of sweet potato from Fujian province were detected by the double antibody enzyme-linked immunosorbent method (DAS-ELISA) .The results showed that 24samples were SPFMV-positive, the positive rate were 47.06%;the highest positive rate was 71.40%, detected in the samples from Quanzhou, and the next were 50.00%, detected in samples from Nanan and Longyan.A pair of primer was designed and synthesized according to the published nucleotide sequence of coat protein gene of SPFMV, and a specific RT-PCR detection procedure was established.The amplified SPFMV-specific fragment in PCR contained 441bps, and showed 92%-97%, homology with the SPFMV reference sequence.
- sweet potato /
- virus /
- sweet potato feathery mottle virus /
- ELISA /
- RT-PCR

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参考文献(14)

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