Effects of Colchicine on Mutagenesis of OncidiumIn Vitro
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摘要: 以秋水仙素为诱变剂, 结合组织培养技术, 对文心兰丛生芽和原球茎进行诱变试验, 并应用分子标记技术检测诱变后代的变异情况。结果表明:培养基添加秋水仙素混合培养法是文心兰丛生芽诱变的最佳途径, 以添加500mg·L-1的秋水仙素诱导10d效果最佳, 植株变异率高达26.7%, 变异植株类型包括植株粗壮、多叶、叶片变宽、变厚、矮化、叶片线艺等;经56次继代培养, 部分变异株系仍保持稳定遗传性状, 其中筛选出的稳定绿叶金边、金叶绿边变异株系RAPD标记表明其在DNA水平上发生了变异, 为文心兰育种提供了新的方法和思路。Abstract: With colchicine was used as the mutagenic agent, combined with tissue culture techniques, the mutagenesis experiments for bud clump and PLB of Oncidium and the molecular marker technology to detect mutagenesis progeny were studied.Resultsshowed that method of mixed medium supplemented with colchicine was the best way to mutage the Oncidium, and the best condition was 10 dinduction with 500 mg·L-1of colchicine.Plant mutation rate was as high as 26.7%, and there were different mutation forms, such as hairchested, polyphyllous, dwarfed plantlets and widened, thickened leaves, and linear, patterned leaves.Mutant strain went through 56successive transfer culture, and part of them remained properties well.Among them, the variation with golden margin leaves and variation with green margin golden leaves had apparent variation compared with the original species on DNA level by RAPD markers.It suggested a new breeding methods and ideas for Oncidium.
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Key words:
- Oncidium /
- culture in vitro /
- protocorm-like body /
- colchicines /
- chemical mutagenesis
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