脂肪酸提取方法对芽胞杆菌种类脂肪酸测定结果的影响

刘国红; 刘波; 唐建阳; 车建美; 朱育菁; 陈峥

doi:10.19303/j.issn.1008-0384.2015.01.010

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脂肪酸提取方法对芽胞杆菌种类脂肪酸测定结果的影响

doi: 10.19303/j.issn.1008-0384.2015.01.010

福建省农业科学院农业生物资源研究所,福建福州,350003

基金项目:

国家自然科学基金项目(31370059)

科技部国际合作项目(2012DFA31120)

农业部948项目(2011-G25)

详细信息

作者简介:
刘国红(1983-),女,博士,主要从事芽胞杆菌分类研究(E-mail:liuguohong624@sina.com);刘波(1957-),男,博士,研究员,主要从事微生物生物技术和农业生物药物研究(E-mail:fzliubo@163.com)

中图分类号: Q936
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- 被引次数: 0
出版历程
- 收稿日期: 2014-08-05
- 刊出日期: 2015-03-18

The Influence of the Fatty Acid Extraction Method on the Fatty Acid Determination of the Bacillus Species

Institute of Agrobiological Resources,Fujian Academy of Agricultural Sciences

摘要

摘要: 脂肪酸是细菌细胞膜的重要组成物质,受细胞膜上遗传物质的控制,具有灵敏度高、成分高度保守和含量稳定等特点,其碳链长度、双键位置和功能团组合的不同使其成为有效的分类标记。在细菌培养和脂肪酸提取阶段,芽胞杆菌属种类的脂肪酸鉴定结果受到皂化时间和脂肪酸提取试剂量的影响。本研究以球形芽胞杆菌为例,分析了皂化时间、皂化试剂量和甲基化试剂量对脂肪酸鉴定结果的影响。研究结果表明:皂化时间在25~30min的测定效果较好;皂化试剂量为1.0mL、甲基化试剂量为1.5mL时,球形芽胞杆菌的脂肪酸鉴定效果较好。
- 芽胞杆菌 /
- 脂肪酸 /
- 提取方法
Abstract: Fatty acids were important components of cell membrane of bacteria,and were subjected to the control of genetic material on cell membrane.The components and contents of fatty acids of Bacillus species were highly conserved and stable.Differences in chain length,position of double bond and binding of functional group,made fatty acids very useful as taxonomic markers.In bacterial culture and fatty acid extraction stages,the experiment results showed that the identification of Bacillus′s fatty acids was affected by saponification time and fatty acid extraction reagent dose.Using type species as object,effects of different detection conditions on fatty acid results of the genus Bacillus were investigated.The optimum conditions for the detection were saponification time of 25 to 30min,saponification reagent dosage of 1.0mL,methylation reagent dosage of 1.5mL.
- Bacillus /
- fatty acids /
- extract method