摘要:
为提高大杯蕈菇柄多糖的得率,缩短提取时间,将大杯蕈菇柄通过加工制成粗粉(20目)、超微粉(300目),以水提醇沉法提取大杯蕈菇柄粗粉和超微粉中的粗多糖,采用苯酚-硫酸法对其粗多糖含量进行测定,研究超微粉碎技术对大杯蕈菇柄多糖溶出效果的影响。结果表明:大杯蕈菇柄超微粉多糖提取的最佳工艺条件为提取时间2h、提取温度80℃、料液比1∶40、提取次数2次,多糖得率8.93%;粗粉多糖的最佳提取条件为提取时间4h、提取温度90℃、料液比1∶40、提取次数1次,多糖得率5.86%。超微粉提取多糖和粗粉相比,提取时间缩短了1/2,多糖得率提高了3.07个百分点,多糖纯度提高了12.14个百分点,体现了省时、高效的优势。超微粉碎技术能显著提高大杯蕈菇柄多糖的溶出度。
关键词:
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大杯蕈菇柄 /
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超微粉碎 /
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多糖 /
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提取
Abstract:
To increase extraction rate in shorted extraction time,microwave-assisted technology was used for extracting polysaccharides of Clitocybe maxima stipe ground by particle size of 20 mesh vs 300 mesh.The crude polysaccharide was extracted from coarse and ultra-fine powder with the solvent water and precipitated with ethanol,then the content was detected using the phenol-sulfuric acid method.The effect on polysaccharide dissolubility was studied for the superfine grinding technology.The results showed that the best extraction parameters for ultra-fine powder were:extraction time 2h,extraction temperature 80℃,the ratio of material to solvent 1∶40,extracting twice,and obtained polysaccharides 8.93%.The extraction parameters for coarse powder were:extraction time 4h,extraction temperature 90℃,the ratio of material to solvent 1∶40,extracting once and polysaccharide yield5.86%.Comparing with coarse powder,extracting polysaccharides with ultra-fine powder shortened extraction time for 50%,increased polysaccharide yield by 3.07 percentage points,and improved the purity of polysaccharides by 12.14 percentage points.It was indicated that ultra-fine grinding technology has improved the extraction efficiency of polysaccharide for Clitocybe Maxima stipe.
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