Effects of Carbon Source and 6-BA on Adventitious Buds Growth and Polysaccharides Accumulation in Polygonatum cyrtonema Hua
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摘要: 探讨不同碳源及6-BA浓度对多花黄精不定芽生长及多糖累积的影响,结果表明:蔗糖比葡萄糖、麦芽糖更适合用于多花黄精不定芽的培养;高浓度6-BA不利于黄精不定芽生长和多糖累积,6-BA浓度应低于1.0mg·L-1;随着蔗糖浓度的增加,折干率和多糖含量也随之增加,结合不定芽的生长速率,认为最适宜多花黄精不定芽培养的蔗糖浓度为80g·L-1。Abstract: In this paper,the effects of different carbon sources and 6-BA concentration on adventitious buds growth and polysaccharide accumulation in P.cyrtonema Hua were studied.Resultsindicated that,sucrose was more suitable for the adventitious buds formation than glucose and maltose.High concentration of 6-BA inhibited the adventitious buds growth and polysaccharide accumulation,and the suitable concentration should be controlled below 1.0 mg·L-1.With the increase of sucrose concentration,dry-weight rate and polysaccharide contention were also increase.Combined with the growth rate,the most suitable sucrose concentration for P.cyrtonema Hua growth was 80g·L-1.
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Key words:
- Polygonatum cyrtonema Hua /
- adventitions bud /
- growth /
- polysaccharide
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