37份药用石斛种质资源亲缘关系的ISSR分析

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37份药用石斛种质资源亲缘关系的ISSR分析

李永清, 江金兰, 叶炜, 雷伏贵, 华树妹

文章导航 > 福建农业学报 > 2015 > 30(2): 131-135

李永清, 江金兰, 叶炜, 雷伏贵, 华树妹. 37份药用石斛种质资源亲缘关系的ISSR分析[J]. 福建农业学报, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

引用本文:

李永清, 江金兰, 叶炜, 雷伏贵, 华树妹. 37份药用石斛种质资源亲缘关系的ISSR分析[J]. 福建农业学报, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

LI Yong-qing, JIANG Jin-lan, YE Wei, LEI Fu-gui, HUA Shu-mei. Relationships Among 37 Germplasm Resources of Medicinal Dendrobium Based on ISSR[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

Citation:

LI Yong-qing, JIANG Jin-lan, YE Wei, LEI Fu-gui, HUA Shu-mei. Relationships Among 37 Germplasm Resources of Medicinal Dendrobium Based on ISSR[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

李永清, 江金兰, 叶炜, 雷伏贵, 华树妹. 37份药用石斛种质资源亲缘关系的ISSR分析[J]. 福建农业学报, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

引用本文:

李永清, 江金兰, 叶炜, 雷伏贵, 华树妹. 37份药用石斛种质资源亲缘关系的ISSR分析[J]. 福建农业学报, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

LI Yong-qing, JIANG Jin-lan, YE Wei, LEI Fu-gui, HUA Shu-mei. Relationships Among 37 Germplasm Resources of Medicinal Dendrobium Based on ISSR[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

Citation:

LI Yong-qing, JIANG Jin-lan, YE Wei, LEI Fu-gui, HUA Shu-mei. Relationships Among 37 Germplasm Resources of Medicinal Dendrobium Based on ISSR[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 131-135. doi: 10.19303/j.issn.1008-0384.2015.02.005

37份药用石斛种质资源亲缘关系的ISSR分析

doi: 10.19303/j.issn.1008-0384.2015.02.005

福建省三明市农业科学研究院药用植物研究所

基金项目:

三明市科技计划项目(2014-N-3)

三明市农业科学院院立项目(2013)

作者简介:
李永清(1980-),男,硕士,助理研究员,主要从事兰科植物育种与生物技术(E-mail:76475275@qq.com);江金兰(1973-),女,高级农艺师,主要从事兰科植物生物技术(E-mail:420465520@qq.com)

中图分类号: S567.239
计量
- 文章访问数: 147
- HTML全文浏览量: 39
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2014-10-05
- 刊出日期: 2015-02-18

Relationships Among 37 Germplasm Resources of Medicinal Dendrobium Based on ISSR

Institute of Medicinal Plant Sciences Sanming Academy of Agricultural Sciences

Funds:

福建省三明市农业科学研究院药用植物研究所,福建沙县,365050

摘要: 利用ISSR技术对37份药用石斛属种质资源进行亲缘关系分析,从备选72条引物中筛选出15条引物,共扩增出89条谱带,有89条多态带,多态性条带比例为100%。采用NTSYS-PC2.0软件分析结果,37份石斛种质资源遗传相似系数在0.50~0.85。聚类结果显示,以相似系数为0.55,可将37份石斛种质明显划分为7个类群,表明药用石斛植物遗传多样性丰富,ISSR技术可应用于药用石斛的亲缘关系研究。
- 石斛 /
- 亲缘关系 /
- ISSR
Abstract: Genetic relationship of 37 Dendrobium was studied by ISSR technique.Fifteen primers with clear and polymorphic bands were screened out from 72 ISSR random primers.Eighty-nine locus were detected with polymorphic percentage in 100%.As analyzed by NTSYS-PC2.0,the genetic similarity of 37 Dendrobium were ranged from 0.50-0.85 and grouped into 7sub-cluster when the similarity coefficient was 0.55.The results indicated that plants of medicinal Dendrobium were rich in genetic diversity and their genetic relationship could be studied by ISSR technique.
- Dendrobium /
- genetic relationship /
- ISSR

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